Evidence map›Paper›PMID 27728978›Full record

ArticleFuture microbiology2017

Inter- and intra-strain variability of tandem repeats in Mycoplasma pneumoniae based on next-generation sequencing data.

Jing Zhang, Xiaohong Song, Marella J Ma, Li Xiao, Tsuyoshi Kenri, Hongmei Sun, Travis Ptacek, Shaoli Li, Ken B Waites, T Prescott Atkinson and 3 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Future microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.5field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Molecular Tools for TypingFrontiers in microbiology · 2022
    Review
  2. Article
  3. Molecular Typing ofJournal of clinical microbiology · 2019
    Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 6 institutions in 3 countries.

Jing ZhangDepartment of Pathogenic Biology, Chongqing Medical University, Chongqing, China.
Xiaohong SongCritical Care Medicine Department, NIH Clinical Center, National Institutes of Health, Bethesda, MD, USA.
Marella J MaWinston Churchill High School, Potomac, MD, USA.
Li XiaoDepartment of Medicine, University of Alabama at Birmingham, AL, USA.
Tsuyoshi KenriDepartment of Bacteriology II, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan.
Hongmei SunDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, China.
Travis PtacekDepartment of Microbiology, University of Alabama at Birmingham, AL, USA.
Shaoli LiDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, China.
Ken B WaitesDepartment of Pathology, University of Alabama at Birmingham, AL, USA.
T Prescott AtkinsonDepartment of Pediatrics, University of Alabama at Birmingham, AL, USA.
Keigo ShibayamaDepartment of Bacteriology II, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan.
Kevin DybvigDepartment of Microbiology, University of Alabama at Birmingham, AL, USA.
Yanmei FengDepartment of Pulmonary Medicine, The First Affiliated Hospital, Chongqing Medical University, Chongqing, China.
University of Alabama at Birmingham · USCapital Institute of Pediatrics · CNNational Institute of Infectious Diseases · JPNational Institutes of Health Clinical Center · USChongqing Medical University · CNWinston Churchill Foundation · US

Funding

UAB Center for Clinical and Translational Science (CCTS)UL1TR001417 · NCATS · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI KIMBERLY, ROBERT P. · 2015 to 2018
$31.4M
Mechanisms of Mycoplasmal Disease PathogenesisR01AI063909 · NIAID · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI DYBVIG, KEVIN F · 2005 to 2014
$3.0M
NCATS NIH HHS UL1 TR001417NIAID NIH HHS R01 AI063909
6 · The paper itself

Abstract

aimTo characterize inter- and intra-strain variability of variable-number tandem repeats (VNTRs) in Mycoplasma pneumoniae to determine the optimal multilocus VNTR analysis scheme for improved strain typing.

methodsWhole genome assemblies and next-generation sequencing data from diverse M. pneumoniae isolates were used to characterize VNTRs and their variability, and to compare the strain discriminability of new VNTR and existing markers.

resultsWe identified 13 VNTRs including five reported previously. These VNTRs displayed different levels of inter- and intra-strain copy number variations. All new markers showed similar or higher discriminability compared with existing VNTR markers and the P1 typing system.

conclusionOur study provides novel insights into VNTR variations and potential new multilocus VNTR analysis schemes for improved genotyping of M. pneumoniae.

Indexed as

Minisatellite RepeatsDNA, BacterialDNA Copy Number VariationsGenetic LociGenetic MarkersGenotyping TechniquesHigh-Throughput Nucleotide SequencingMycoplasma pneumoniaeSequence Analysis, DNADNA, BacterialGenetic MarkersMycoplasma pneumoniaenext-generation sequencingtandem repeats

Identifiers

PMID27728978
PMCPMC5551932
OpenAlexW2531552252

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.