Evidence map›Paper›PMID 27728810›Full record

ArticleBiomaterials2016

Exosomes as biomimetic tools for stem cell differentiation: Applications in dental pulp tissue regeneration.

Chun-Chieh Huang, Raghuvaran Narayanan, Satish Alapati, Sriram Ravindran

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 187 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
187citing papers in PubMed, 3 pooled it
8.0field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

187 citing papers in PubMed, 3 syntheses or guidelines pooled it, 314 citations in OpenAlex.

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  17. Isolation methods of exosomes derived from dental stem cells.International journal of oral science · 2025
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127 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Chun-Chieh HuangDepartment of Oral Biology, University of Illinois at Chicago, USA.
Raghuvaran NarayananDepartment of Oral Biology, University of Illinois at Chicago, USA.
Satish AlapatiDepartment of Endodontics, University of Illinois at Chicago, USA.
Sriram RavindranDepartment of Oral Biology, University of Illinois at Chicago, USA. Electronic address: sravin1@uic.edu.
University of Illinois Chicago · US

Funding

ECM MEDIATED DENTAL PULP TISSUE REGENERATIONR56DE023806 · NIDCR · UNIVERSITY OF ILLINOIS AT CHICAGO · PI ALAPATI, SATISH B, RAVINDRAN, SRIRAM · 2014 to 2014
$400k
NIDCR NIH HHS R56 DE023806
6 · The paper itself

Abstract

Achieving and maintaining safe and reliable lineage specific differentiation of stem cells is important for clinical translation of tissue engineering strategies. In an effort to circumvent the multitude of problems arising from the usage of growth factors and growth factor delivery systems, we have explored the use of exosomes as biomimetic tools to induce stem cell differentiation. Working on the hypothesis that cell-type specific exosomes can trigger lineage-specific differentiation of stem cells, we have evaluated the potential of exosomes derived from dental pulp cells cultured on under growth and odontogenic differentiation conditions to induce odontogenic differentiation of naïve human dental pulp stem cells (DPSCs) and human bone marrow derived stromal cells (HMSCs) in vitro and in vivo. Results indicate that the exosomes can bind to matrix proteins such as type I collagen and fibronectin enabling them to be tethered to biomaterials. The exosomes are endocytosed by both DPSCs and HMSCs in a dose-dependent and saturable manner via the caveolar endocytic mechanism and trigger the P38 mitogen activated protein kinase (MAPK) pathway. In addition, the exosomes also trigger the increased expression of genes required for odontogenic differentiation. When tested in vivo in a tooth root slice model with DPSCs, the exosomes triggered regeneration of dental pulp-like tissue. However, our results indicate that exosomes isolated under odontogenic conditions are better inducers of stem cell differentiation and tissue regeneration. Overall, our results highlight the potential exosomes as biomimetic tools to induce lineage specific differentiation of stem cells. Our results also show the importance of considering the source and state of exosome donor cells before a choice is made for therapeutic applications.

Indexed as

Biomimetic MaterialsCell DifferentiationCells, CulturedDental PulpExosomesHumansOdontogenesisRegenerationStem CellsBiomimeticsDental pulp regenerationDental pulp stem cellsExosomesRegenerative endodontics

Identifiers

PMID27728810
PMCPMC5293278
OpenAlexW2526445427

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.