ArticleDevelopmental cell2016
Comparative Principles of DNA Methylation Reprogramming during Human and Mouse In Vitro Primordial Germ Cell Specification.
Article in Developmental cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 66 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
66 citing papers in PubMed, 1 synthesis or guideline pooled it, 116 citations in OpenAlex.
- Metabolic transitions define spermatogonial stem cell maturation.Human reproduction (Oxford, England) · 2022Pooled it
- Endocrinology at a Miniature Level: Pluripotent Stem-Cell-Derived Organoid Models of Hypothalamus-Pituitary Axes.Biomolecules · 2026Review
- The road to restore male fertility using in vitro-derived germ cells.Human reproduction update · 2026Review
- Exiting the ground state: the broad spectrum of cell fates accessible from naïve human pluripotent stem cells.Cellular and molecular life sciences : CMLS · 2026Review
- Generating human primordial germ cell-like cells from pluripotent stem cells: a scoping review ofHuman reproduction open · 2025Review
- Epigenetic reprogramming in mouse and human primordial germ cells.Experimental & molecular medicine · 2024Review
- Article
- The Establishment and Optimization of a Chicken Primordial Germ Cell Induction Model Using Small-Molecule Compounds.Animals : an open access journal from MDPI · 2024Article
- Modeling X-chromosome inactivation and reactivation during human development.Current opinion in genetics & development · 2023Review
- Epigenetic dynamics during capacitation of naïve human pluripotent stem cells.Science advances · 2023Article
- The role of mtDNA in oocyte quality and embryo development.Molecular reproduction and development · 2023Review
- Transgenerational inheritance and its modulation by environmental cues.Current topics in developmental biology · 2023Article
- Current progress onFrontiers in cell and developmental biology · 2023Review
- Mouse primordial germ-cell-like cells lack piRNAs.Developmental cell · 2022Article
- Bend family proteins mark chromatin boundaries and synergistically promote early germ cell differentiation.Protein & cell · 2022Article
- An Interplay between Epigenetics and Translation in Oocyte Maturation and Embryo Development: Assisted Reproduction Perspective.Biomedicines · 2022Review
- BMP4 drives primed to naïve transition through PGC-like state.Nature communications · 2022Article
- A hominoid-specific endogenous retrovirus may have rewired the gene regulatory network shared between primordial germ cells and naïve pluripotent cells.PLoS genetics · 2022Article
- Article
- Interplay between chromatin marks in development and disease.Nature reviews. Genetics · 2022Review
6 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
Primordial germ cell (PGC) development is characterized by global epigenetic remodeling, which resets genomic potential and establishes an epigenetic ground state. Here we recapitulate PGC specification in vitro from naive embryonic stem cells and characterize the early events of epigenetic reprogramming during the formation of the human and mouse germline. Following rapid de novo DNA methylation during priming to epiblast-like cells, methylation is globally erased in PGC-like cells. Repressive chromatin marks (H3K9me2/3) and transposable elements are enriched at demethylation-resistant regions, while active chromatin marks (H3K4me3 or H3K27ac) are more prominent at regions that demethylate faster. The dynamics of specification and epigenetic reprogramming show species-specific differences, in particular markedly slower reprogramming kinetics in the human germline. Differences in developmental kinetics may be explained by differential regulation of epigenetic modifiers. Our work establishes a robust and faithful experimental system of the early events of epigenetic reprogramming and regulation in the germline.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.