ArticlePLoS neglected tropical diseases2016
The Mitochondrial Genomes of the Zoonotic Canine Filarial Parasites Dirofilaria (Nochtiella) repens and Candidatus Dirofilaria (Nochtiella) hongkongensis Provide Evidence for Presence of Cryptic Species.
Article in PLoS neglected tropical diseases, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 31 papers, 1 of them a synthesis that pooled it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 65 citations in OpenAlex.
- Medical Parasitology Taxonomy Update, 2016-2017.Journal of clinical microbiology · 2019Pooled it
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- Ghosts of symbionts past: the hidden history of the dynamic association between filarial nematodes and their Wolbachia endosymbionts.G3 (Bethesda, Md.) · 2025Article
- Circulation ofLife (Basel, Switzerland) · 2025Article
- Genetic characterization of zoonoticMicrobiology spectrum · 2025Article
- Unveiling Dirofilaria Asiatica infection: first clinical insights and treatment challenges for this feline zoonotic parasitosis.Veterinary research communications · 2025Article
- Vector-borne pathogens in dogs from the Republic of Kosovo.Parasites & vectors · 2025Article
- Zoonotic Dirofilaria sp. "hongkongensis" in subcutaneous nodules from dogs and cats, Hong Kong SAR.Parasites & vectors · 2024Article
- A filarial parasite potentially associated with the health burden on domestic chickens in Japan.Scientific reports · 2024Article
- Development and validation of a long-read metabarcoding platform for the detection of filarial worm pathogens of animals and humans.BMC microbiology · 2024Article
- Whole genome analysis of two sympatric humanFrontiers in cellular and infection microbiology · 2023Article
- A case of humanDermatology reports · 2022Article
- A study on canine dirofilariasis in selected areas of Sri Lanka.BMC research notes · 2022Article
- What lies behind the curtain: Cryptic diversity in helminth parasites of human and veterinary importance.Current research in parasitology & vector-borne diseases · 2022Review
- Emergence ofVeterinary world · 2021Article
- Heartworm disease - Overview, intervention, and industry perspective.International journal for parasitology. Drugs and drug resistance · 2021Review
- Human and Animal Dirofilariasis in Southeast of France.Microorganisms · 2021Article
- Genetic diversity of vector-borne pathogens in spotted and brown hyenas from Namibia and Tanzania relates to ecological conditions rather than host taxonomy.Parasites & vectors · 2021Article
- Article
- Molecular Analysis of Dirofilaria repens Isolates from Eye-Care Patients in Greece.Acta parasitologica · 2021Article
Corrections and comments
- Erratum issued
Authors and funding
15 authors at 11 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCutaneous dirofilariosis is a canine mosquito-borne zoonosis that can cause larva migrans disease in humans. Dirofilaria repens is considered an emerging pathogen occurring with high prevalence in Mediterranean areas and many parts of tropical Asia. In Hong Kong, a second species, Candidatus Dirofilaria hongkongensis, has been reported. The present study aimed to compare mitochondrial genomes from these parasites and to obtain population genetic information. METHODS AND
findingsComplete mitochondrial genomes were obtained by PCR and Sanger sequencing or ILLUMINA sequencing for four worms. Cytochrome oxidase subunit 1 sequences identified three as D. repens (all from Europe) and one as C. D. hongkongensis (from India). Mitochondrial genomes have the same organization as in other spirurid nematodes but a higher preference for thymine in the coding strand. Phylogenetic analysis was in contradiction to current taxonomy of the Onchocercidae but in agreement with a recent multi-locus phylogenetic analysis using both mitochondrial and nuclear markers. D. repens and C. D. hongkongensis sequences clustered together and were the common sister group to Dirofilaria immitis. Analysis of a 2.5 kb mitochondrial genome fragment from macrofilaria or canine blood samples from Europe (42), Thailand (2), India (1) and Vietnam (1) revealed only small genetic differences in the D. repens samples including all European and the Vietnam sample. The Indian C. D. hongkongensis and the two Thai samples formed separate clusters and differences were comparatively large.
conclusionGenetic differences between Dirofilaria spp. causing cutaneous disease can be considerable whereas D. repens itself was genetically quite homogenous. C. D. hongkongensis was identified for the first time from the Indian subcontinent. The full mitochondrial genome sequence strengthens the hypothesis that it represents an independent species and the Thai samples might represent another cryptic species, Candidatus Dirofilaria sp. 'Thailand II', or a quite divergent population of C. D. hongkongensis.
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