Effects of oral administration of whole plants of Artemisia annua on Ichthyophthirius multifiliis and Aeromonas hydrophila after parasitism by I. multifiliis.

Since the use of malachite green was banned, it is desirable to search for alternative drug to control Ichthyophthirius multifiliis infection. Moreover, route of administration which is easy to implement and less stressful to fish is important to treat infection of fish with this parasite. It is also important to enhance protective effect against Aeromonas hydrophila infection after parasitism by I. multifiliis. The present study evaluated the protective effect of diet supplemented with Artemisia annua against I. multifiliis and A. hydrophila infection after the parasitism. The results showed that oral administration with A. annua at a concentration of 20 g/kg feed for 45 days can provide strong protection against I. multifiliis infection. The incidence of infestation and mean number of trophonts on fish fins on day 3 post-challenge (15,000 theronts/fish) were significantly reduced compared with the control, and the survival rate of fish during 15-day period was also increased by nearly 30 %. But, this diet provided very limited protection against higher infection dose. Besides, this study demonstrated that this diet enhanced protective effect against A. hydrophila after the fish were infected with I. multifiliis. On the basis of the above results, oral administration with A. annua can be used as a potential strategy for control I. multifiliis infection and increasing fish survival after parasitism by I. multifiliis.