ArticleAmerican journal of translational research2016
BMP2 promotes the differentiation of neural stem cells into dopaminergic neurons
Article in American journal of translational research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Controlled release of bone morphogenetic protein-2 improves motor function after traumatic brain injury in a rat model.Journal of materials chemistry. B · 2026Article
- A Critical Review of Commercial Collagen-Based Scaffolds in Bone Regeneration: Functional Properties and Clinical Evidence from InfuseJournal of functional biomaterials · 2025Review
- Hsa_circ_0054220 Upregulates HMGA1 by the Competitive RNA Pattern to Promote Neural Impairment in MPTP Model of Parkinson's Disease.Applied biochemistry and biotechnology · 2024Article
- Adult Neurogenesis of the Medial Geniculate Body: In Vitro and Molecular Genetic Analyses Reflect the Neural Stem Cell Capacity of the Rat Auditory Thalamus over Time.International journal of molecular sciences · 2024Article
- Directional induction of neural stem cells, a new therapy for neurodegenerative diseases and ischemic stroke.Cell death discovery · 2023Review
- Biomaterial application strategies to enhance stem cell-based therapy for ischemic stroke.World journal of stem cells · 2022Article
- Inhibition of miR-145-5p Reduces Spinal Cord Injury-Induced Inflammatory and Oxidative Stress Responses via Affecting Nurr1-TNF-α Signaling Axis.Cell biochemistry and biophysics · 2021Article
- Emerging roles of microRNAs in intestinal ischemia/reperfusion-induced injury: a review.Journal of physiology and biochemistry · 2020Review
- The potential of bone morphogenetic protein 2 as a neurotrophic factor for Parkinson's disease.Neural regeneration research · 2020Review
- Change of Nurr1 expression in mouse hippocampal CA3 region following excitotoxic neuronal damage.Iranian journal of basic medical sciences · 2020Article
- Altered Nurr1 protein expression in the hippocampal CA1 region following transient global cerebral ischemia.Molecular medicine reports · 2020Article
- Inhibition of lysine-specific demethylase 1A suppresses neointimal hyperplasia by targeting bone morphogenetic protein 2 and mediating vascular smooth muscle cell phenotype.Cell proliferation · 2020Article
- miR-381-3p knockdown improves intestinal epithelial proliferation and barrier function after intestinal ischemia/reperfusion injury by targeting nurr1.Cell death & disease · 2018Article
- Epigenetic Regulations in Neural Stem Cells and Neurological Diseases.Stem cells international · 2018Review
- Targeting bone morphogenetic protein signalling in midbrain dopaminergic neurons as a therapeutic approach in Parkinson's disease.Neuronal signaling · 2017Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNeural stem cells (NSCs) are pluripotent and self-renewing cells which could differentiate into diverse types of neural cells, such as dopaminergic (DA) neurons, the loss of which is the typical characteristic of Parkinson's disease (PD). This study aimed to examine the molecular mechanisms of BMP2-mediating NSCs differentiation into DA neurons.
methodsDifferent concentrations of BMP2 were used to induce the differentiation of NSCs into DA neurons, which were characterized by the number and the neurite lengths of tyrosine hydroxylase (TH)+ and dopamine transporter (DAT)+ neurons by immunocytochemistry. qRT-PCR and Western blot were performed to explore the expression of miR-145 and Nurr1. The methylation level of miR-145 promoter was examined by DNA methylation analyses. The regulation of miR-145 on Nurr1 was detected by Dual-Luciferase reporter assay.
resultsThe number of TH+ and DAT+ neurons were significantly increased in NSCs treated with 20 and 100 ng/ml of BMP2, as well as the neurite lengths of TH+ and DAT+ neurons. The reduced level of miR-145 and up-regulated Nurr1 were observed in NSCs induced by BMP2. The hypermethylation level of miR-145 promoter down-regulated the expression of miR-145 in NSCs pretreated with BMP2, which was regulated by DNMT3b. Luciferase reporter assay showed that Nurr1 was a direct target of miR-145. miR-145 overexpression restrained the differentiating effect of BMP2. Moreover, overexpression of Nurr1 abrogated this effect of miR-145 overexpression.
conclusionOur results showed that BMP2 promoted the differentiation of NSCs into DA neurons
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27725851PMC5040669What OpenQuestion holds
Registered trials
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