ArticleScientific reports2016
Cleaning patch-clamp pipettes for immediate reuse.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed.
- DuBois-Reymond prize lecture 2024: Multi-neuron patch-clamp to uncover the neuronal and synaptic physiology in the human neocortex.Pflugers Archiv : European journal of physiology · 2026Article
- Mutation E300 recommended by protein language models gives ChrimsonR amplified photocurrent response.bioRxiv : the preprint server for biology · 2026Article
- High-throughput multiplex voltage-clamp/current-clamp evaluation of acutely isolated neurons.Nature protocols · 2026Review
- Electrophysiological classification of human layer 2-3 pyramidal neurons reveals subtype-specific synaptic interactions.Nature neuroscience · 2026Article
- The evolution of patch-clamp electrophysiology: Robotic, multiplex, and dynamic.Molecular pharmacology · 2025Review
- Role of Neural Circuits in Cognitive Impairment.Neurochemical research · 2024Review
- Patch-walking, a coordinated multi-pipette patch clamp for efficiently finding synaptic connections.eLife · 2024Article
- ARViS: a bleed-free multi-site automated injection robot for accurate, fast, and dense delivery of virus to mouse and marmoset cerebral cortex.Nature communications · 2024Article
- Patch-walking: Coordinated multi-pipette patch clamp for efficiently finding synaptic connections.bioRxiv : the preprint server for biology · 2024Article
- Immediate reuse of patch-clamp pipettes after ultrasonic cleaning.Scientific reports · 2024Article
- Electrophysiological evaluation of the effect of peptide toxins on voltage-gated ion channels: a scoping review on theoretical and methodological aspects with focus on the Central and South American experience.The journal of venomous animals and toxins including tropical diseases · 2024Article
- Electrophysiological and morphological characterization of single neurons in intact human brain organoids.Journal of neuroscience methods · 2023Article
- Article
- High-throughput combined voltage-clamp/current-clamp analysis of freshly isolated neurons.Cell reports methods · 2023Article
- Current approaches to characterize micro- and macroscale circuit mechanisms of Parkinson's disease in rodent models.Experimental neurology · 2022Review
- Automated microscope-independent fluorescence-guided micropipette.Biomedical optics express · 2021Article
- Method for Rapid Enzymatic Cleaning for Reuse of Patch Clamp Pipettes: Increasing Throughput by Eliminating Manual Pipette Replacement between Patch Clamp Attempts.Bio-protocol · 2021Article
- Automated Intracellular Pharmacological Electrophysiology for Ligand-Gated Ionotropic Receptor and Pharmacology Screening.Molecular pharmacology · 2021Article
- Deep learning-based real-time detection of neurons in brain slices for in vitro physiology.Scientific reports · 2021Article
- In Vivo Whole-Cell Patch-Clamp Methods: Recent Technical Progress and Future Perspectives.Sensors (Basel, Switzerland) · 2021Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Patch-clamp recording has enabled single-cell electrical, morphological and genetic studies at unparalleled resolution. Yet it remains a laborious and low-throughput technique, making it largely impractical for large-scale measurements such as cell type and connectivity characterization of neurons in the brain. Specifically, the technique is critically limited by the ubiquitous practice of manually replacing patch-clamp pipettes after each recording. To circumvent this limitation, we developed a simple, fast, and automated method for cleaning glass pipette electrodes that enables their reuse within one minute. By immersing pipette tips into Alconox, a commercially-available detergent, followed by rinsing, we were able to reuse pipettes 10 times with no degradation in signal fidelity, in experimental preparations ranging from human embryonic kidney cells to neurons in culture, slices, and in vivo. Undetectable trace amounts of Alconox remaining in the pipette after cleaning did not affect ion channel pharmacology. We demonstrate the utility of pipette cleaning by developing the first robot to perform sequential patch-clamp recordings in cell culture and in vivo without a human operator.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.