ArticleCell transplantation2017
Effect of Gelatin on Osteogenic Cell Sheet Formation Using Canine Adipose-Derived Mesenchymal Stem Cells.
Article in Cell transplantation, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 71 citations in OpenAlex.
- Interpenetrating gelatin/sodium alginate hydrogel with controlled PRP lysate delivery for accelerating diabetic wound healing with reduced scarring.RSC advances · 2026Article
- Scaffold-free cell sheet therapies: clinical advances, global approval landscapes, and strategic directions to address regenerative medicine barriers.Stem cell research & therapy · 2025Review
- Advanced therapeutic scaffolds of biomimetic periosteum for functional bone regeneration.Journal of nanobiotechnology · 2025Review
- Mechanical harvesting of cell sheets: an efficient approach for bone and cartilage tissue engineering.Stem cell research & therapy · 2025Review
- Novel Applications of Natural Biomaterials in Dentistry-Properties, Uses, and Development Perspectives.Materials (Basel, Switzerland) · 2025Review
- Rheological Characterization and Printability of Sodium Alginate-Gelatin Hydrogel for 3D Cultures and Bioprinting.Biomimetics (Basel, Switzerland) · 2025Article
- Alkaline shear-thinning micro-nanocomposite hydrogels initiate endogenous TGFβ signaling for in situ bone regeneration.NPJ Regenerative medicine · 2023Article
- Hydrogel-Forming Microneedles with Applications in Oral Diseases Management.Materials (Basel, Switzerland) · 2023Review
- New Photocrosslinked 3D Foamed Scaffolds Based on GelMA Copolymers: Potential Application in Bone Tissue Engineering.Gels (Basel, Switzerland) · 2023Article
- Application of mesenchymal stem cell sheet for regeneration of craniomaxillofacial bone defects.Stem cell research & therapy · 2023Review
- Optimization of 3D printing andFrontiers in bioengineering and biotechnology · 2023Article
- Construction of biomimetic cell-sheet-engineered periosteum with a double cell sheet to repair calvarial defects of rats.Journal of orthopaedic translation · 2023Article
- Surfactant-free gelatin-stabilised biodegradable polymerised high internal phase emulsions with macroporous structures.Frontiers in chemistry · 2023Article
- 142Development of an alginate-gelatin bioink enhancing osteogenic differentiation by gelatin release.International journal of bioprinting · 2023Article
- Biodegradable Hydrogel Beads Combined with Calcium Phosphate Bone Cement for Bone Repair: In Vitro and In Vivo Characterization.Polymers · 2022Article
- Effects of carrier solutions on the viability and efficacy of canine adipose-derived mesenchymal stem cells.BMC veterinary research · 2022Article
- 3D-Printed Gelatin-Alginate Hydrogel Dressings for Burn Wound Healing: A Comprehensive Study.International journal of bioprinting · 2022Article
- Cell Sheet Technology as an Engineering-Based Approach to Bone Regeneration.International journal of nanomedicine · 2022Review
- Bioprinting of Stem Cells in Multimaterial Scaffolds and Their Applications in Bone Tissue Engineering.Sensors (Basel, Switzerland) · 2021Review
- In Vitro Evaluation of Calcium Phosphate Bone Cement Composite Hydrogel Beads of Cross-Linked Gelatin-Alginate with Gentamicin-Impregnated Porous Scaffold.Pharmaceuticals (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Osteogenically differentiated cell sheet techniques using mesenchymal stem cells (MSCs) are available to stimulate bone regeneration. The advantage of the cell sheet technique is delivering live cells effectively into the focal region. We developed a novel osteogenic cell sheet technique by adding gelatin to osteogenic cell medium. Gelatin-induced osteogenic cell sheets (GCSs) were compared to conventional osteogenic cell sheets (OCSs). Undifferentiated MSCs (UCs) were used as a control. The morphology of these cell sheets was evaluated microscopically and histologically. The time-dependent cell proliferation rate was estimated by DNA quantification. The expression of osteogenic gene markers and the number of calcium depositions were assessed by quantitative real-time polymerase chain reaction and Alizarin red S (ARS) staining, respectively. GCSs were thicker and stronger than OCSs. GCSs showed a significantly higher cell proliferation rate compared to OCSs (p < 0.05). GCSs exhibited significantly higher upregulation of BMP-7 mRNA compared to OCSs (p < 0.05). Both GCSs and OCSs showed negative ARS reactivity on day 10, but only GCSs showed positive ARS reactivity on day 21. With this technique, we observed active cell proliferation with abundant ECM and upregulation of osteogenic bone markers, and our results suggest that GCSs could be promising for therapeutic applications in bone regeneration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.