ArticleIntegrative biology : quantitative biosciences from nano to macro2016
High content image analysis of focal adhesion-dependent mechanosensitive stem cell differentiation.
Article in Integrative biology : quantitative biosciences from nano to macro, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- How Actin Polymerization and Myosin II Activity Regulate Focal Adhesion Dynamics in Motile Cells.International journal of molecular sciences · 2025Article
- Hippo in smooth muscle - a therapeutic target in vascular diseases driven by aging and hypertension.Frontiers in physiology · 2025Review
- Role of mechano-sensitive non-coding RNAs in bone remodeling of orthodontic tooth movement: recent advances.Progress in orthodontics · 2022Review
- Nuclear mechanoprotection: From tissue atlases as blueprints to distinctive regulation of nuclear lamins.APL bioengineering · 2022Review
- A Review on the Design of Hydrogels With Different Stiffness and Their Effects on Tissue Repair.Frontiers in bioengineering and biotechnology · 2022Review
- Long Non-coding RNAs LOC100126784 and POM121L9P Derived From Bone Marrow Mesenchymal Stem Cells Enhance Osteogenic Differentiation via the miR-503-5p/SORBS1 Axis.Frontiers in cell and developmental biology · 2021Article
- Mechano-responsiveness of fibrillar adhesions on stiffness-gradient gels.Journal of cell science · 2020Article
- Identification of Key Signaling Pathways Orchestrating Substrate Topography Directed Osteogenic Differentiation Through High-Throughput siRNA Screening.Scientific reports · 2019Article
- Vinexin family (SORBS) proteins regulate mechanotransduction in mesenchymal stem cells.Scientific reports · 2018Article
- Automated Buildup of Biomimetic Films in Cell Culture Microplates for High-Throughput Screening of Cellular Behaviors.Advanced materials (Deerfield Beach, Fla.) · 2018Article
- Cell-Extracellular Matrix Mechanobiology: Forceful Tools and Emerging Needs for Basic and Translational Research.Nano letters · 2018Article
- Understanding the extracellular forces that determine cell fate and maintenance.Development (Cambridge, England) · 2017Review
- Vinexin family (SORBS) proteins play different roles in stiffness-sensing and contractile force generation.Journal of cell science · 2017Article
- Stem cell migration and mechanotransduction on linear stiffness gradient hydrogels.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Omics Approaches to Identify Potential Biomarkers of Inflammatory Diseases in the Focal Adhesion Complex.Genomics, proteomics & bioinformatics · 2017Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Human mesenchymal stem cells (hMSCs) receive differentiation cues from a number of stimuli, including extracellular matrix (ECM) stiffness. The pathways used to sense stiffness and other physical cues are just now being understood and include proteins within focal adhesions. To rapidly advance the pace of discovery for novel mechanosensitive proteins, we employed a combination of in silico and high throughput in vitro methods to analyze 47 different focal adhesion proteins for cryptic kinase binding sites. High content imaging of hMSCs treated with small interfering RNAs for the top 6 candidate proteins showed novel effects on both osteogenic and myogenic differentiation; Vinculin and SORBS1 were necessary for stiffness-mediated myogenic and osteogenic differentiation, respectively. Both of these proteins bound to MAPK1 (also known as ERK2), suggesting that it plays a context-specific role in mechanosensing for each lineage; validation for these sites was performed. This high throughput system, while specifically built to analyze stiffness-mediated stem cell differentiation, can be expanded to other physical cues to more broadly assess mechanical signaling and increase the pace of sensor discovery.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.