ArticleNature structural & molecular biology2016
Regulation of CED-3 caspase localization and activation by C. elegans nuclear-membrane protein NPP-14.
Article in Nature structural & molecular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 15 citations in OpenAlex.
- Endogenous expression and subcellular localization of core apoptosis regulators reveal key differences between embryonic and germline apoptosis in C. elegans.Cell death and differentiation · 2026Article
- Endogenous Expression and Subcellular Localization of Core Apoptosis Regulators Reveal Key Differences Between Embryonic and Germline Apoptosis in C. elegans.Research square · 2026Article
- Fluorescent protein tagging of C. elegans core apoptosis pathway components reveals mitochondrial localization of CED-9 Bcl-2, CED-4 Apaf1 and CED-3 Caspase in non-apoptotic and apoptotic cells.Cell death and differentiation · 2026Article
- Staphylococcus aureus-induced degeneration of nociceptive neurons in Caenorhabditis elegans.BMC biology · 2025Article
- A nuclear pore-anchored condensate enables germ granule organization and transgenerational epigenetic inheritance.Nature structural & molecular biology · 2025Article
- Article
- Interaction between DLC-1 and SAO-1 facilitates CED-4 translocation during apoptosis in the Caenorhabditis elegans germline.Cell death discovery · 2022Article
- TUNEL Labeling to Detect Double-stranded DNA Breaks inBio-protocol · 2022Article
- Analysis of Nuclear Pore Complexes in Caenorhabditis elegans by Live Imaging and Functional Genomics.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The nuclear envelope: target and mediator of the apoptotic process.Cell death discovery · 2020Review
- Discovery of small molecule inhibitors for the C. elegans caspase CED-3 by high-throughput screening.Biochemical and biophysical research communications · 2017Article
- Direct pro-apoptotic role for NPM1 as a regulator of PIDDosome formation.Molecular & cellular oncology · 2017Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Caspases are cysteine proteases with critical roles in apoptosis. The Caenorhabditis elegans caspase CED-3 is activated by autocatalytic cleavage, a process enhanced by CED-4. Here we report that the CED-3 zymogen localizes to the perinuclear region in C. elegans germ cells and that CED-3 autocatalytic cleavage is held in check by C. elegans nuclei and activated by CED-4. The nuclear-pore protein NPP-14 interacts with the CED-3 zymogen prodomain, colocalizes with CED-3 in vivo and inhibits CED-3 autoactivation in vitro. Several missense mutations in the CED-3 prodomain result in stronger association with NPP-14 and decreased CED-3 activation by CED-4 in the presence of nuclei or NPP-14, thus leading to cell-death defects. Those same mutations enhance autocatalytic cleavage of CED-3 in vitro and increase apoptosis in vivo in the absence of npp-14. Our results reveal a critical role of nuclei and nuclear-membrane proteins in regulating the activation and localization of CED-3.
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