ArticleACS chemical biology2016
Domain Organization and Active Site Architecture of a Polyketide Synthase C-methyltransferase.
Article in ACS chemical biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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Who cites it
30 citing papers in PubMed, 49 citations in OpenAlex.
- Deciphering the Function and Structure of PA1216 as an S-Adenosyl-l-Methionine Binding Protein Using Differential Scanning Fluorimetry and Circular Dichroism.Protein science : a publication of the Protein Society · 2026Article
- Evolution, structure and function of the putative biosynthetic gene cluster of the fungal secondary metabolite myriocin, a potent inhibitory sphingolipid.Communications biology · 2026Article
- Stereochemical Dissection of the Strobilurin PKS Reveals the Complex Biosynthetic Logic of Iterative EZE Triene Construction.Angewandte Chemie (International ed. in English) · 2026Article
- Methyltransferase Domain-Focused Genome Mining for Fungal Polyketide Synthases.Small methods · 2024Article
- Structure of a modular polyketide synthase reducing region.Structure (London, England : 1993) · 2023Article
- Crosstalk between primary and secondary metabolism: Interconnected fatty acid and polyketide biosynthesis in prokaryotes.Bioorganic & medicinal chemistry letters · 2023Review
- Biosynthesis of the Isocoumarin Derivatives Fusamarins is Mediated by the PKS8 Gene Cluster in Fusarium.Chembiochem : a European journal of chemical biology · 2023Article
- Anammox BacterialInternational journal of molecular sciences · 2023Article
- Thiamyxins: Structure and Biosynthesis of Myxobacterial RNA-Virus Inhibitors.Angewandte Chemie (International ed. in English) · 2022Article
- Structural Basis for Control of Methylation Extent in Polyketide Synthase Metal-DependentACS chemical biology · 2022Article
- Prospecting for natural products by genome mining and microcrystal electron diffraction.Nature chemical biology · 2021Article
- Fatty Acid Biosynthesis in Chromerids.Biomolecules · 2020Article
- Site directed mutagenesis as a precision tool to enable synthetic biology with engineered modular polyketide synthases.Synthetic and systems biotechnology · 2020Review
- Molecular basis of methylation and chain-length programming in a fungal iterative highly reducing polyketide synthase.Chemical science · 2019Article
- Insight into Structural Characteristics of Protein-Substrate Interaction in Pimaricin Thioesterase.International journal of molecular sciences · 2019Article
- Structural and Functional Studies of a gem-Dimethylating Methyltransferase from a trans-Acyltransferase Assembly Line.ACS chemical biology · 2018Article
- Structural Basis of Polyketide Synthase O-Methylation.ACS chemical biology · 2018Article
- Exploring Fungal Polyketide C-Methylation through Combinatorial Domain Swaps.ACS chemical biology · 2018Article
- Protein-protein interactions in "cis-AT" polyketide synthases.Natural product reports · 2018Review
- The architectures of iterative type I PKS and FAS.Natural product reports · 2018Review
Corrections and comments
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Authors and funding
7 authors at 6 institutions in 1 country.
Funding
Abstract
Polyketide metabolites produced by modular type I polyketide synthases (PKS) acquire their chemical diversity through the variety of catalytic domains within modules of the pathway. Methyltransferases are among the least characterized of the catalytic domains common to PKS systems. We determined the domain boundaries and characterized the activity of a PKS C-methyltransferase (C-MT) from the curacin A biosynthetic pathway. The C-MT catalyzes S-adenosylmethionine-dependent methyl transfer to the α-position of β-ketoacyl substrates linked to acyl carrier protein (ACP) or a small-molecule analog but does not act on β-hydroxyacyl substrates or malonyl-ACP. Key catalytic residues conserved in both bacterial and fungal PKS C-MTs were identified in a 2 Å crystal structure and validated biochemically. Analysis of the structure and the sequences bordering the C-MT provides insight into the positioning of this domain within complete PKS modules.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.