ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2017
Rice NICOTIANAMINE SYNTHASE 2 expression improves dietary iron and zinc levels in wheat.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers, 1 of them a synthesis that pooled it.
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Who cites it
41 citing papers in PubMed, 1 synthesis or guideline pooled it, 126 citations in OpenAlex.
- Meta-QTL analysis of seed iron and zinc concentration and content in common bean (Phaseolus vulgaris L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2018Pooled it
- Analysis of the TaNAS gene family in bread wheat identifies additional members and candidates for intragenic biofortification.Journal of experimental botany · 2026Article
- From paddy soil to dining table: biological biofortification of rice with zinc.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Review
- Identification of the Nicotianamine synthase (NAS) gene family in wheat (Triticum aestivum L.) and the role of its member TaNAS4-A in Zn and Fe transport.Functional & integrative genomics · 2025Article
- Article
- Micronutrient Biofortification in Wheat: QTLs, Candidate Genes and Molecular Mechanism.International journal of molecular sciences · 2025Review
- Unlocking Opportunities and Overcoming Challenges in Genetically Engineered Biofortification.Nutrients · 2025Review
- Biofortification of baby corn: Integrating agronomic and genetic approaches to address zinc micronutrient deficiencies - global perspectives and future challenges.Frontiers in plant science · 2025Review
- Article
- Biofortification of Triticum species: a stepping stone to combat malnutrition.BMC plant biology · 2024Review
- Nicotianamine: A Key Player in Metal Homeostasis and Hyperaccumulation in Plants.International journal of molecular sciences · 2023Review
- Arabidopsis nicotianamine synthases comprise a common core-NAS domain fused to a variable autoinhibitory C terminus.The Journal of biological chemistry · 2023Article
- Proof of concept and early development stage of market-oriented high iron and zinc rice expressing dicot ferritin and rice nicotianamine synthase genes.Scientific reports · 2023Article
- A two-gene strategy increases iron and zinc concentrations in wheat flour, improving mineral bioaccessibility.Plant physiology · 2023Article
- Biofortification to avoid malnutrition in humans in a changing climate: Enhancing micronutrient bioavailability in seed, tuber, and storage roots.Frontiers in plant science · 2023Review
- Overexpression ofPlants (Basel, Switzerland) · 2022Article
- Pleiotropic effect analysis and marker development for grain zinc and iron concentrations in spring wheat.Molecular breeding : new strategies in plant improvement · 2022Article
- Genotypic differences in response of durum wheat (Plant direct · 2022Article
- Genomic approaches for improving grain zinc and iron content in wheat.Frontiers in genetics · 2022Review
- Strategies and Bottlenecks in Hexaploid Wheat to Mobilize Soil Iron to Grains.Frontiers in plant science · 2022Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageIron and zinc deficiencies negatively impact human health worldwide. We developed wheat lines that meet or exceed recommended dietary target levels for iron and zinc in the grains. These lines represent useful germplasm for breeding new wheat varieties that can reduce iron and zinc deficiency-associated health burdens in the affected populations. Micronutrient deficiencies, including iron and zinc deficiencies, have negative impacts on human health globally. Iron-deficiency; anemia affects nearly two billion people worldwide and is the cause of reduced cognitive development, fatigue and overall low productivity. Similarly, zinc deficiency causes stunted growth, decreased immunity and increased risk of respiratory infections. Biofortification of staple crops is a sustainable and effective approach to reduce the burden of health problems associated with micronutrient deficiencies. Here, we developed wheat lines expressing rice NICOTIANAMINE SYNTHASE 2 (OsNAS2) and bean FERRITIN (PvFERRITIN) as single genes as well as in combination. NAS catalyzes the biosynthesis of nicotianamine (NA), which is a precursor of the iron chelator deoxymugeneic acid (DMA) required for long distance iron translocation. FERRITIN is important for iron storage in plants because it can store up to 4500 iron ions. We obtained significant increases of iron and zinc content in wheat grains of plants expressing either OsNAS2 or PvFERRTIN, or both genes. In particular, wheat lines expressing OsNAS2 greatly surpass the HarvestPlus recommended target level of 30 % dietary estimated average requirement (EAR) for iron, and 40 % of EAR for zinc, with lines containing 93.1 µg/g of iron and 140.6 µg/g of zinc in the grains. These wheat lines with dietary significant levels of iron and zinc represent useful germplasm for breeding new wheat varieties that can reduce micronutrient deficiencies in affected populations.
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