ArticleCell discovery2016
ASK1 controls spindle orientation and positioning by phosphorylating EB1 and stabilizing astral microtubules.
Article in Cell discovery, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- ENKD1 Modulates Skin Elasticity Through Microtubule Stability Regulation.Cytoskeleton (Hoboken, N.J.) · 2026Article
- SAPCD2 phosphorylation modulates astral microtubule stability to control spindle orientation.Communications biology · 2026Article
- Dynein Light Intermediate Chains Exhibit Different Arginine Methylation Patterns.Journal of clinical laboratory analysis · 2024Article
- Understanding the underlying mechanisms governing spindle orientation: How far are we from there?Journal of cellular and molecular medicine · 2022Review
- The specialized mitotic behavior of human embryonic stem cells.Cell and tissue research · 2022Article
- Redox-dependent regulation of end-binding protein 1 activity by glutathionylation.Science China. Life sciences · 2021Article
- Alteration of cell junctions during viral infection.Thoracic cancer · 2020Review
- Orientation of the Mitotic Spindle in Blood Vessel Development.Frontiers in cell and developmental biology · 2020Review
- A New Quantitative Cell-Based Assay Reveals Unexpected Microtubule Stabilizing Activity of Certain Kinase Inhibitors, Clinically Approved or in the Process of Approval.Frontiers in pharmacology · 2020Article
- Insulin Induces Microtubule Stabilization and Regulates the Microtubule Plus-end Tracking Protein Network in Adipocytes.Molecular & cellular proteomics : MCP · 2019Article
- Non-canonical functions of the mitotic kinesin Eg5.Thoracic cancer · 2018Review
- Exopolysaccharides from a Codonopsis pilosula endophyte activate macrophages and inhibit cancer cell proliferation and migration.Thoracic cancer · 2018Article
- High-performance gene expression and knockout tools using sleeping beauty transposon system.Mobile DNA · 2018Article
- Histone deacetylase 6 modulates macrophage infiltration during inflammation.Theranostics · 2018Article
- EB1 phosphorylation mediates the functions of ASK1 in pancreatic cancer development.Oncotarget · 2017Article
- Akap350 Recruits Eb1 to The Spindle Poles, Ensuring Proper Spindle Orientation and Lumen Formation in 3d Epithelial Cell Cultures.Scientific reports · 2017Article
- Effects of FSTL1 on the proliferation and motility of breast cancer cells and vascular endothelial cells.Thoracic cancer · 2017Article
- Regulation of end-binding protein EB1 in the control of microtubule dynamics.Cellular and molecular life sciences : CMLS · 2017Review
- Phosphorylation of EB1 regulates the recruitment of CLIP-170 and p150glued to the plus ends of astral microtubules.Oncotarget · 2017Article
- Apoptosis-linked gene 2 promotes breast cancer growth and metastasis by regulating the cytoskeleton.Oncotarget · 2017Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Orientation and positioning of the mitotic spindle are involved in dictating cell division axis and cleavage site, and play important roles in cell fate determination and tissue morphogenesis. However, how spindle movement is controlled to achieve a defined alignment within the dividing cell is not fully understood. Here, we describe an unexpected role for apoptosis signal-regulating kinase 1 (ASK1) in regulating spindle behavior. We find that ASK1 is required for proper mitotic progression and daughter cell adhesion to the substratum. ASK1 interacts with end-binding protein 1 (EB1) and phosphorylates EB1 at serine 40, threonine 154 and threonine 206, enhancing its binding to the plus ends of astral microtubules. Consequently, astral microtubules are stabilized and therefore capable of mediating spindle interaction with the cell cortex, a requirement for spindle movement. These findings reveal a previously undiscovered function of ASK1 in cell division by regulating spindle orientation and positioning, and point to the importance of protein phosphorylation in the regulation of spindle behavior.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.