ArticleMetabolomics : Official journal of the Metabolomic Society2016
Inaccurate quantitation of palmitate in metabolomics and isotope tracer studies due to plastics.
Article in Metabolomics : Official journal of the Metabolomic Society, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed.
- A protocol for noninvasive quantification of dietary fat absorption in mice.STAR protocols · 2026Article
- Secretory phospholipase ACommunications biology · 2025Article
- NFIA regulates articular chondrocyte fatty acid metabolism and joint homeostasis.Science translational medicine · 2025Article
- Sample Preparation for Metabolomic Analysis in Exercise Physiology.Biomolecules · 2024Review
- Identification of Metabolite Interference Is Necessary for Accurate LC-MS Targeted Metabolomics Analysis.Analytical chemistry · 2023Article
- Using mass spectrometry imaging to map fluxes quantitatively in the tumor ecosystem.Nature communications · 2023Article
- Quantitative Determination of De Novo Fatty Acid Synthesis in Brown Adipose Tissue Using Deuterium Oxide.Journal of visualized experiments : JoVE · 2023Article
- Uncoupled glycerol-3-phosphate shuttle in kidney cancer reveals that cytosolic GPD is essential to support lipid synthesis.Molecular cell · 2023Article
- Sequential lipidomic, metabolomic, and proteomic analyses of serum, liver, and heart tissue specimens from peroxisomal biogenesis factor 11α knockout mice.Analytical and bioanalytical chemistry · 2022Article
- Thermodynamic Genome-Scale Metabolic Modeling of Metallodrug Resistance in Colorectal Cancer.Cancers · 2021Article
- Article
- Profiling of mouse macrophage lipidome using direct infusion shotgun mass spectrometry.STAR protocols · 2021Article
- Development of an optimized sample preparation method for quantification of free fatty acids in food using liquid chromatography-mass spectrometry.Scientific reports · 2021Article
- Targeting unique biological signals on the fly to improve MS/MS coverage and identification efficiency in metabolomics.Analytica chimica acta · 2021Article
- Comprehensive metabolic profiling of Parkinson's disease by liquid chromatography-mass spectrometry.Molecular neurodegeneration · 2021Article
- Improved Annotation of Untargeted Metabolomics Data through Buffer Modifications That Shift Adduct Mass and Intensity.Analytical chemistry · 2020Article
- Recommendations and Best Practices for Standardizing the Pre-Analytical Processing of Blood and Urine Samples in Metabolomics.Metabolites · 2020Review
- Lipidomic Phenotyping Reveals Extensive Lipid Remodeling during Adipogenesis in Human Adipocytes.Metabolites · 2020Article
- The key points in the pre-analytical procedures of blood and urine samples in metabolomics studies.Metabolomics : Official journal of the Metabolomic Society · 2020Review
- Chemical Discovery in the Era of Metabolomics.Journal of the American Chemical Society · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
introductionPalmitate, the typical end product released from fatty acid synthase, is of interest to many researchers performing metabolomics. Although palmitate can be readily detected by using mass spectrometry, many metabolomic platforms involve the use of plastic consumables that introduce a competing background signal of palmitate.
objectivesThe goal of this study was to quantify palmitate contamination in metabolomics and isotope tracer studies and to examine the reliability of approaches for reducing error.
methodsWe measured the quantitative error introduced by palmitate contamination from 4 vendors of plastic consumables used in combination with several different extraction solvents.
resultsThe background palmitate signal was as much as sixfold higher than the biological palmitate signal from 4 million 3T3-L1 cells. Importantly, the palmitate contamination signal was highly variable between plastic consumables (even within the same lot) and therefore could not be accurately removed by subtracting the background as measured from a blank. In addition to affecting relative and absolute quantitation, the palmitate background signal from disposable plastics also led to the underestimation of labeled palmitate in isotope tracer experiments.
conclusionWhen measuring palmitate standard solutions, the best results were obtained when glass vials and glass pipettes were used. However, much of the palmitate background signal could be eliminated by pre-rinsing plastic vials and plastic pipette tips with methanol prior to sample introduction. For isotope tracer studies, error could also be minimized by estimating palmitate enrichment from palmitoylcarnitine, which does not have a competing contamination signal from plastic consumables.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.