ArticleJournal of microbiological methods2016
Direct loop mediated isothermal amplification on filters for quantification of Dehalobacter in groundwater.
Article in Journal of microbiological methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed, 13 citations in OpenAlex.
- Critical review of methods for isothermal amplification of nucleic acids for environmental analysis.Journal of microbiological methods · 2020Review
- Rapid detection of cytochrome cd1-containing nitrite reductase encoding gene nirS of denitrifying bacteria with loop-mediated isothermal amplification assay.Scientific reports · 2020Article
- Microfluidic-Based Nucleic Acid Amplification Systems in Microbiology.Micromachines · 2019Review
- On-filter direct amplification of Legionella pneumophila for rapid assessment of its abundance and viability.Water research · 2017Article
- Quantification of microRNAs directly from body fluids using a base-stacking isothermal amplification method in a point-of-care device.Biomedical microdevices · 2017Article
- Isothermal assay targeting class 1 integrase gene for environmental surveillance of antibiotic resistance markers.Journal of environmental management · 2017Article
- Implications of direct amplification for measuring antimicrobial resistance using point-of-care devices.Analytical methods : advancing methods and applications · 2017Article
- Recommendations for developing and applying genetic tools to assess and manage biological invasions in marine ecosystems.Marine policy · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 2 countries.
Funding
Abstract
Nucleic acid amplification of biomarkers is increasingly used to monitor microbial activity and assess remedial performance in contaminated aquifers. Previous studies described the use of filtration, elution, and direct isothermal amplification (i.e. no DNA extraction and purification) as a field-able means to quantify Dehalococcoides spp. in groundwater. This study expands previous work with direct loop mediated isothermal amplification (LAMP) for the detection and quantification of Dehalobacter spp. in groundwater. Experiments tested amplification of DNA with and without crude lysis and varying concentrations of humic acid. Three separate field-able methods of biomass concentration with eight aquifer samples were also tested, comparing direct LAMP with traditional DNA extraction and quantitative PCR (qPCR). A new technique was developed where filters were amplified directly within disposable Gene-Z chips. The direct filter amplification (DFA) method eliminated an elution step and provided a detection limit of 10
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.