ArticlePrenatal diagnosis2016
Optimization of techniques for multiple platform testing in small, precious samples such as human chorionic villus sampling.
Article in Prenatal diagnosis, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Current approaches and developments in transcript profiling of the human placenta.Human reproduction update · 2020Pooled it
- Differences in First-Trimester Maternal Metabolomic Profiles in Pregnancies Conceived From Fertility Treatments.The Journal of clinical endocrinology and metabolism · 2019Trial
- The identification of challenges in tissue collection for biomarker studies: the Q-CROC-03 neoadjuvant breast cancer translational trial experience.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2017Trial
- Leveraging chorionic villus biopsies for the derivation of patient-specific trophoblast stem cells.Communications biology · 2025Article
- RNA degradation patterns in cardiac tissues kept at different time intervals and temperatures before RNA sequencing.PloS one · 2025Article
- Sexually dimorphic DNA methylation and gene expression patterns in human first trimester placenta.Biology of sex differences · 2024Article
- High-throughput mRNA-seq atlas of human placenta shows vast transcriptome remodeling from first to third trimester†.Biology of reproduction · 2024Article
- Sex differences in microRNA expression in first and third trimester human placenta†.Biology of reproduction · 2022Article
- High-throughput miRNA sequencing of the human placenta: expression throughout gestation.Epigenomics · 2021Article
- Sexually Dimorphic Crosstalk at the Maternal-Fetal Interface.The Journal of clinical endocrinology and metabolism · 2020Article
- Differential gene expression during placentation in pregnancies conceived with different fertility treatments compared with spontaneous pregnancies.Fertility and sterility · 2019Article
- Hemochorial placentation: development, function, and adaptations.Biology of reproduction · 2018Review
- Sex differences in the late first trimester human placenta transcriptome.Biology of sex differences · 2018Article
- Up-regulation of microRNA-202-3p in first trimester placenta of pregnancies destined to develop severe preeclampsia, a pilot study.Pregnancy hypertension · 2017Article
Corrections and comments
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Authors and funding
14 authors.
Funding
Abstract
backgroundMultiple testing to understand global changes in gene expression based on genetic and epigenetic modifications is evolving. Chorionic villi, obtained for prenatal testing, is limited, but can be used to understand ongoing human pregnancies. However, optimal storage, processing and utilization of CVS for multiple platform testing have not been established.
resultsLeftover CVS samples were flash-frozen or preserved in RNAlater. Modifications to standard isolation kits were performed to isolate quality DNA and RNA from samples as small as 2-5 mg. RNAlater samples had significantly higher RNA yields and quality and were successfully used in microarray and RNA-sequencing (RNA-seq). RNA-seq libraries generated using 200 versus 800-ng RNA showed similar biological coefficients of variation. RNAlater samples had lower DNA yields and quality, which improved by heating the elution buffer to 70 °C. Purification of DNA was not necessary for bisulfite-conversion and genome-wide methylation profiling. CVS cells were propagated and continue to express genes found in freshly isolated chorionic villi.
conclusionsCVS samples preserved in RNAlater are superior. Our optimized techniques provide specimens for genetic, epigenetic and gene expression studies from a single small sample which can be used to develop diagnostics and treatments using a systems biology approach in the prenatal period. © 2016 John Wiley & Sons, Ltd.
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