ArticleMicrobial cell factories2016
Metabolic engineering of Corynebacterium glutamicum for enhanced production of 5-aminovaleric acid.
Article in Microbial cell factories, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 115 citations in OpenAlex.
- Metabolic engineering of Corynebacterium glutamicum for high-yield de novo biosynthesis of 5-aminovaleramide, a promising bio-based monomer.Microbial cell factories · 2026Article
- Construction of a genome-engineered stable 5-aminolevulinic acid producingSynthetic and systems biotechnology · 2025Article
- Metabolic Engineering of Corynebacterium glutamicum for High-Level Production of 1,5-Pentanediol, a C5 Diol Platform Chemical.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Recent advances in the Design-Build-Test-Learn (DBTL) cycle for systems metabolic engineering of Corynebacterium glutamicum.Journal of microbiology (Seoul, Korea) · 2025Review
- Novel Bioproduction of 1,6-Hexamethylenediamine from l-Lysine Based on an Artificial One-Carbon Elongation Cycle.ACS omega · 2024Article
- Targeted and high-throughput gene knockdown in diverse bacteria using synthetic sRNAs.Nature communications · 2023Article
- Production of γ-Aminobutyrate (GABA) in RecombinantACS omega · 2022Article
- Rebooting life: engineering non-natural nucleic acids, proteins and metabolites in microorganisms.Microbial cell factories · 2022Review
- Metabolic Engineering ofMicroorganisms · 2022Article
- Targeted Metabolomic Analysis in Alzheimer's Disease Plasma and Brain Tissue in Non-Hispanic Whites.Journal of Alzheimer's disease : JAD · 2022Article
- Metabolic Engineering for Valorization of Agri- and Aqua-Culture Sidestreams for Production of Nitrogenous Compounds byFrontiers in microbiology · 2022Review
- Exploring functionality of the reverse β-oxidation pathway in Corynebacterium glutamicum for production of adipic acid.Microbial cell factories · 2021Article
- A Genome-Scale Metabolic Model ofMetabolites · 2021Article
- Recent progress in production of amino acid-derived chemicals using Corynebacterium glutamicum.World journal of microbiology & biotechnology · 2021Review
- Evaluation of Heterologous Biosynthetic Pathways for Methanol-Based 5-Aminovalerate Production by ThermophilicFrontiers in bioengineering and biotechnology · 2021Article
- A High-Efficiency Artificial Synthetic Pathway for 5-Aminovalerate Production From Biobased L-Lysine inFrontiers in bioengineering and biotechnology · 2021Article
- Genomic and Transcriptomic Investigation of the Physiological Response of the MethylotrophFrontiers in microbiology · 2021Article
- Coproduction of 5-Aminovalerate and δ-Valerolactam for the Synthesis of Nylon 5 From L-Lysine inFrontiers in bioengineering and biotechnology · 2021Article
- Glutaric acid production by systems metabolic engineering of an l-lysine-overproducingProceedings of the National Academy of Sciences of the United States of America · 2020Article
- FermentativeMicroorganisms · 2020Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background5-Aminovaleric acid (5AVA) is an important five-carbon platform chemical that can be used for the synthesis of polymers and other chemicals of industrial interest. Enzymatic conversion of L-lysine to 5AVA has been achieved by employing lysine 2-monooxygenase encoded by the davB gene and 5-aminovaleramidase encoded by the davA gene. Additionally, a recombinant Escherichia coli strain expressing the davB and davA genes has been developed for bioconversion of L-lysine to 5AVA. To use glucose and xylose derived from lignocellulosic biomass as substrates, rather than L-lysine as a substrate, we previously examined direct fermentative production of 5AVA from glucose by metabolically engineered E. coli strains. However, the yield and productivity of 5AVA achieved by recombinant E. coli strains remain very low. Thus, Corynebacterium glutamicum, a highly efficient L-lysine producing microorganism, should be useful in the development of direct fermentative production of 5AVA using L-lysine as a precursor for 5AVA. Here, we report the development of metabolically engineered C. glutamicum strains for enhanced fermentative production of 5AVA from glucose.
resultsVarious expression vectors containing different promoters and origins of replication were examined for optimal expression of Pseudomonas putida davB and davA genes encoding lysine 2-monooxygenase and delta-aminovaleramidase, respectively. Among them, expression of the C. glutamicum codon-optimized davA gene fused with His
conclusionsCorynebacterium glutamicum was successfully engineered to produce 5AVA from glucose by optimizing the expression of two key enzymes, lysine 2-monooxygenase and delta-aminovaleramidase. In addition, production of glutaric acid, a major byproduct, was significantly reduced by employing C. glutamicum gabT mutant as a host strain. The metabolically engineered C. glutamicum strains developed in this study should be useful for enhanced fermentative production of the novel C5 platform chemical 5AVA from renewable resources.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.