ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2016
Telomere content measurement in human hematopoietic cells: Comparative analysis of qPCR and Flow-FISH techniques.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed.
- Quantifying telomere length: from bulk assays to single-molecule resolution.Biophysics reports · 2026Article
- Novel Cell Quantification Method Using a Single Surrogate Calibration Curve Across Various Biological Samples.The AAPS journal · 2023Article
- Method comparison studies of telomere length measurement using qPCR approaches: A critical appraisal of the literature.PloS one · 2021Article
- Cell cycle-dependent and -independent telomere shortening accompanies murine brain aging.Aging · 2018Article
- Telomere attrition in heart failure: a flow-FISH longitudinal analysis of circulating monocytes.Journal of translational medicine · 2018Article
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8 authors.
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Abstract
Abnormal telomere lengths have been linked to cancer and other hematologic disorders. Determination of mean telomere content (MTC) is traditionally performed by Southern blotting and densitometry, giving a mean telomere restriction fragment (TRF) value for the total cell population studied. Here, we compared a quantitative Polymerase Chain Reaction approach (qPCR) and a flow cytometric approach, fluorescence in situ hybridization (Flow-FISH), to evaluate telomere content distribution in total patient peripheral blood mononuclear cells or specific cell populations. Flow-FISH is based on in situ hybridization using a fluorescein-labeled peptide nucleic acid (PNA) (CCCTAA)
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