ArticleMicrobial cell factories2016
Lambda Red recombinase-mediated integration of the high molecular weight DNA into the Escherichia coli chromosome.
Article in Microbial cell factories, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 40 citations in OpenAlex.
- Development of an efficient genome editing system using recombinase-mediated cassette exchange inSynthetic and systems biotechnology · 2026Article
- Bacterial population dynamics during colonization of solid tumors.Molecular systems biology · 2026Article
- Bacteriophages mobilize bacterial defense systems via lateral transduction.Science advances · 2026Article
- Emerging trends in genome integration tools for precision engineering of diverse bacterial species.Synthetic biology (Oxford, England) · 2025Review
- CsiR-Mediated Signal Transduction Pathway in Response to Low Iron Conditions Promotes Escherichia coli K1 Invasion and Penetration of the Blood-Brain Barrier.The Journal of infectious diseases · 2024Article
- AJournal of bacteriology · 2024Article
- Plasmid-free cheater cells commonly evolve during laboratory growth.Applied and environmental microbiology · 2024Article
- AbioRxiv : the preprint server for biology · 2024Article
- Recent advances in genome-scale engineering inEngineering microbiology · 2024Review
- Mobile and Self-Sustained Data Storage in an Extremophile Genomic DNA.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Robust counterselection and advanced λRed recombineering enable markerless chromosomal integration of large heterologous constructs.Nucleic acids research · 2022Article
- YbdO Promotes the Pathogenicity ofInternational journal of molecular sciences · 2022Article
- RpoE Facilitates Stress-Resistance, Invasion, and Pathogenicity ofMicroorganisms · 2022Article
- Rcs phosphorelay affects the sensitivity ofFrontiers in microbiology · 2022Article
- Developing Multi-Copy Chromosomal Integration Strategies for Heterologous Biosynthesis of Caffeic Acid inFrontiers in microbiology · 2022Article
- Outer Membrane Channel Protein TolC Regulates Escherichia coli K12 Sensitivity to Plantaricin BM-1 via the CpxR/CpxA Two-Component Regulatory System.Probiotics and antimicrobial proteins · 2021Article
- YbfA Regulates the Sensitivity ofFrontiers in microbiology · 2021Article
- EngineeredExperimental and therapeutic medicine · 2020Review
- Homology-dependent recombination of large synthetic pathways into E. coli genome via λ-Red and CRISPR/Cas9 dependent selection methodology.Microbial cell factories · 2020Article
- The Effects of Lactose Induction on a Plasmid-FreeBioengineering (Basel, Switzerland) · 2020Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundEscherichia coli K-12 is a frequently used host for a number of synthetic biology and biotechnology applications and chassis for the development of the minimal cell factories. Novel approaches for integrating high molecular weight DNA into the E. coli chromosome would therefore greatly facilitate engineering efforts in this bacterium.
resultsWe developed a reliable and flexible lambda Red recombinase-based system, which utilizes overlapping DNA fragments for integration of the high molecular weight DNA into the E. coli chromosome. Our chromosomal integration strategy can be used to integrate high molecular weight DNA of variable length into any non-essential locus in the E. coli chromosome. Using this approach we integrated 15 kb DNA encoding sucrose catabolism and lactose metabolism and transport operons into the fliK locus of the flagellar region 3b in the E. coli K12 MG1655 chromosome. Furthermore, with this system we integrated 50 kb of Bacillus subtilis 168 DNA into two target sites in the E. coli K12 MG1655 chromosome. The chromosomal integrations into the fliK locus occurred with high efficiency, inhibited motility, and did not have a negative effect on the growth of E. coli.
conclusionsIn addition to the rational design of synthetic biology devices, our high molecular weight DNA chromosomal integration system will facilitate metabolic and genome-scale engineering of E. coli.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.