ArticleBMC biology2016
Generation of an integrated Hieracium genomic and transcriptomic resource enables exploration of small RNA pathways during apomixis initiation.
Article in BMC biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
- Article
- Apomixis: oh, what a tangled web we have!Planta · 2023Review
- Apomixis: genetic basis and controlling genes.Horticulture research · 2022Article
- The emerging role of small RNAs in ovule development, a kind of magic.Plant reproduction · 2021Review
- Single-cell transcriptome profiling of buffelgrass (Cenchrus ciliaris) eggs unveils apomictic parthenogenesis signatures.Scientific reports · 2021Article
- A study of the heterochronic sense/antisense RNA representation in florets of sexual and apomictic Paspalum notatum.BMC genomics · 2021Article
- Article
- Sexual modulation in a polyploid grass: a reproductive contest between environmentally inducible sexual and genetically dominant apomictic pathways.Scientific reports · 2020Article
- Article
- Review
- Small RNA-seq reveals novel regulatory components for apomixis in Paspalum notatum.BMC genomics · 2019Article
- A brief note on genes that trigger components of apomixis.Journal of biosciences · 2019Review
- Phenotypic plasticity of aposporous embryo sac development inPlant signaling & behavior · 2019Article
- Comparative transcriptome analysis of the wild-type model apomict Hieracium praealtum and its loss of parthenogenesis (lop) mutant.BMC plant biology · 2018Article
- Asexual Female Gametogenesis Involves Contact with a Sexually-Fated Megaspore in ApomicticPlant physiology · 2018Article
- Temporal and spatial expression of genes involved in DNA methylation during reproductive development of sexual and apomictic Eragrostis curvula.Scientific reports · 2017Article
- Integument cell gelatinisation-the fate of the integumentary cells in Hieracium and Pilosella (Asteraceae).Protoplasma · 2017Article
Corrections and comments
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Authors and funding
13 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundApplication of apomixis, or asexual seed formation, in crop breeding would allow rapid fixation of complex traits, economizing improved crop delivery. Identification of apomixis genes is confounded by the polyploid nature, high genome complexity and lack of genomic sequence integration with reproductive tissue transcriptomes in most apomicts.
resultsA genomic and transcriptomic resource was developed for Hieracium subgenus Pilosella (Asteraceae) which incorporates characterized sexual, apomictic and mutant apomict plants exhibiting reversion to sexual reproduction. Apomicts develop additional female gametogenic cells that suppress the sexual pathway in ovules. Disrupting small RNA pathways in sexual Arabidopsis also induces extra female gametogenic cells; therefore, the resource was used to examine if changes in small RNA pathways correlate with apomixis initiation. An initial characterization of small RNA pathway genes within Hieracium was undertaken, and ovary-expressed ARGONAUTE genes were identified and cloned. Comparisons of whole ovary transcriptomes from mutant apomicts, relative to the parental apomict, revealed that differentially expressed genes were enriched for processes involved in small RNA biogenesis and chromatin silencing. Small RNA profiles within mutant ovaries did not reveal large-scale alterations in composition or length distributions; however, a small number of differentially expressed, putative small RNA targets were identified.
conclusionsThe established Hieracium resource represents a substantial contribution towards the investigation of early sexual and apomictic female gamete development, and the generation of new candidate genes and markers. Observed changes in small RNA targets and biogenesis pathways within sexual and apomictic ovaries will underlie future functional research into apomixis initiation in Hieracium.
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