ArticleBMC microbiology2016
Crystal structure and DNA binding activity of a PadR family transcription regulator from hypervirulent Clostridium difficile R20291.
Article in BMC microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 18 citations in OpenAlex.
- The effects of PstR, a PadR family transcriptional regulatory factor, in Plesiomonas shigelloides are revealed by transcriptomics.BMC microbiology · 2024Article
- Identification ofJournal of bacteriology · 2024Article
- Genome-scale identification and comparative analysis of transcription factors in thermophilic cyanobacteria.BMC genomics · 2024Article
- Clostridioides difficile Modifies its Aromatic Compound Metabolism in Response to Amidochelocardin-Induced Membrane Stress.mSphere · 2022Article
- Agr Quorum Sensing influences the Wood-Ljungdahl pathway in Clostridium autoethanogenum.Scientific reports · 2022Article
- Transcriptome analysis unveils survival strategies of Streptococcus parauberis against fish serum.PloS one · 2021Article
- Characterization and overproduction of cell-associated cholesterol oxidase ChoD from Streptomyces lavendulae YAKB-15.Scientific reports · 2019Article
- Prevalence and Diversity Analysis of Candidate Prophages to Provide An Understanding on Their Roles inViruses · 2019Article
- Structural basis of effector and operator recognition by the phenolic acid-responsive transcriptional regulator PadR.Nucleic acids research · 2017Article
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Authors and funding
6 authors at 1 institution in 1 country.
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Abstract
backgroundClostridium difficile is a spore-forming obligate anaerobe that can remain viable for extended periods, even in the presence of antibiotics, which contributes to the persistence of this bacterium as a human pathogen during host-to-host transmission and in hospital environments. We examined the structure and function of a gene product with the locus tag CDR20291_0991 (cdPadR1) as part of our broader goal aimed at elucidating transcription regulatory mechanisms involved in virulence and antibiotic resistance of the recently emergent hypervirulent C. difficile strain R20291. cdPadR1 is genomically positioned near genes that are involved in stress response and virulence. In addition, it was previously reported that cdPadR1 and a homologue from the historical C. difficile strain 630 (CD630_1154) were differentially expressed when exposed to stressors, including antibiotics.
resultsThe crystal structure of cdPadR1 was determined to 1.9 Å resolution, which revealed that it belongs to the PadR-s2 subfamily of PadR transcriptional regulators. cdPadR1 binds its own promoter and other promoter regions from within the C. difficile R20291 genome. DNA binding experiments demonstrated that cdPadR1 binds a region comprised of inverted repeats and an AT-rich core with the predicted specific binding motif, GTACTAT(N
conclusionsOur results indicate that cdPadR1 binds a region within its own promoter consisting of the binding motif GTACTAT(N
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