Evidence map›Paper›PMID 27714590›Full record

ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2017

Technical and clinical validation of three commercial real-time PCR kits for the diagnosis of neuroborreliosis in cerebrospinal fluid on three different real-time PCR platforms.

L Maes, T Carolus, V De Preter, S Ignoul, R Cartuyvels, L Braeken, P-J D'Huys, V Saegeman, B Kabamba, M Raymaekers

Abstract readValidation Study
PubMed Publisher
In one paragraph

Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Analytical comparison of 11 CE-IVD marked real-time PCR assays for Borrelia burgdorferi sensu lato detection.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2025
    Article
  2. Evaluation of six TaqMan RT-rtPCR kits on two thermocyclers for the reliable detection of rabies virus RNA.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 1 country.

L MaesResearch group Lab4U, Group of Management and Technology, UC Leuven-Limburg, Agoralaan gebouw B bus 3, 3590, Diepenbeek, Belgium. liesbeth.maes@ucll.be.
T CarolusResearch group Lab4U, Group of Management and Technology, UC Leuven-Limburg, Agoralaan gebouw B bus 3, 3590, Diepenbeek, Belgium.
V De PreterResearch group Lab4U, Group of Management and Technology, UC Leuven-Limburg, Agoralaan gebouw B bus 3, 3590, Diepenbeek, Belgium.
S IgnoulResearch group Lab4U, Group of Management and Technology, UC Leuven-Limburg, Agoralaan gebouw B bus 3, 3590, Diepenbeek, Belgium.
R CartuyvelsClinical Biology, Laboratory for Molecular Diagnostics, Campus Salvator, Jessa Hospital, Salvatorstraat 20, 3500, Hasselt, Belgium.
L BraekenResearch group Lab4U, Faculty of Industrial Engineering, KU Leuven - Campus Diepenbeek, Agoralaan gebouw B, 3590, Diepenbeek, Belgium.
P-J D'HuysResearch group Lab4U, Group of Management and Technology, UC Leuven-Limburg, Agoralaan gebouw B bus 3, 3590, Diepenbeek, Belgium.
V SaegemanDepartment of Laboratory Medicine, University Hospitals Leuven, Leuven, Belgium.
B KabambaClinical Microbiology, Laboratory of Microbiology, University Clinic St-Luc UCL, Avenue Mounier 10 F, 1200, Brussels, Belgium.
M RaymaekersClinical Biology, Laboratory for Molecular Diagnostics, Campus Salvator, Jessa Hospital, Salvatorstraat 20, 3500, Hasselt, Belgium.
University Colleges Leuven-Limburg · BEJessa Hospital · BECliniques Universitaires Saint-Luc · BEHasselt University · BEKU Leuven · BE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study reports the evaluation of the technical and clinical validation of the O-DiaBorburg kit (DIA), Borrelia burgdorferi PCR kit, ISEX (GENE), and Borrelia burgdorferi sensu lato Real-TM (SAC) for the diagnosis of neuroborreliosis in cerebrospinal fluid based on both Borrelia DNA and CSF samples from patients with clinical suspicion of neuroborreliosis. This validation study was done by analysing the kits on the Rotorgene Q (RGQ), CFX96, and LightCycler480 (LC480). For all kits, the linear range was larger on RGQ than on CFX96 and LC480. A good reproducibility was obtained for all assays on all instruments. Storage at -20 °C resulted in a decreased reproducibility for SAC. Results of the limit of detection (LOD

Indexed as

AdolescentAdultAgedAged, 80 and overBorrelia burgdorferi GroupCerebrospinal FluidChildChild, PreschoolFemaleHumansLyme NeuroborreliosisMaleMiddle AgedMolecular Diagnostic TechniquesReal-Time Polymerase Chain ReactionReproducibility of Results

Identifiers

PMID27714590
OpenAlexW2530914919

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.