ArticleScientific reports2016
A Nucleotide Signature for the Identification of Angelicae Sinensis Radix (Danggui) and Its Products.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Advances in the Identification Methods of Food-Medicine Homologous Herbal Materials.Foods (Basel, Switzerland) · 2025Review
- Rapid authentication of endangeredFrontiers in plant science · 2025Article
- Integrating DNA Barcoding Within an Orthogonal Approach for Herbal Product Authentication: A Narrative Review.Phytochemical analysis : PCA · 2025Review
- DNA barcoding in herbal medicine: Retrospective and prospective.Journal of pharmaceutical analysis · 2023Review
- A specific SNP-based multiplex PCR assay for the simultaneous identification of two biological ingredients for the Chinese patent medicine, Danggui Buxue pill.Frontiers in pharmacology · 2023Article
- DNA barcoding: an efficient technology to authenticate plant species of traditional Chinese medicine and recent advances.Chinese medicine · 2022Review
- A nucleotide signature for the identification of Pinelliae Rhizoma (Banxia) and its products.Molecular biology reports · 2022Article
- Development of a Genus-Universal Nucleotide Signature for the Identification and Supervision ofMolecules (Basel, Switzerland) · 2022Article
- Detection of Adulteration and Pesticide Residues in Chinese Patent Medicine Qipi Pill Using KASP Technology and GC-MS/MS.Frontiers in nutrition · 2022Article
- Mitochondrial Function as a Potential Tool for Assessing Function, Quality and Adulteration in Medicinal Herbal Teas.Frontiers in pharmacology · 2021Review
- Specific DNA mini-barcoding for identification ofChinese medicine · 2020Article
- DetectingGenes · 2019Article
- Mitigating the Impact of Admixtures in Thai Herbal Products.Frontiers in pharmacology · 2019Review
- DNA Mini-Barcoding: A Derived Barcoding Method for Herbal Molecular Identification.Frontiers in plant science · 2019Review
- Rapid Authentication of Ginkgo biloba Herbal Products Using the Recombinase Polymerase Amplification Assay.Scientific reports · 2018Article
- Detection of Cistanches Herba (Frontiers in plant science · 2018Article
- Medicinal Materials DNA Barcode Database (MMDBD) version 1.5-one-stop solution for storage, BLAST, alignment and primer design.Database : the journal of biological databases and curation · 2018Article
- Decision Tree Algorithm-Generated Single-Nucleotide Polymorphism Barcodes ofEvolutionary bioinformatics online · 2018Article
- Method for the Identification of Taxon-SpecificFrontiers in plant science · 2018Article
- Establishment of the most comprehensive ITS2 barcode database to date of the traditional medicinal plant Rhodiola (Crassulacaee).Scientific reports · 2017Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is very difficult to identify Angelicae sinensis radix (Danggui) when it is processed into Chinese patent medicines. The proposed internal transcribed spacer 2 (ITS2) is not sufficient to resolve heavily processed materials. Therefore, a short barcode for the identification of processed materials is urgently needed. In this study, 265 samples of Angelicae sinensis radix and adulterants were collected. The ITS2 region was sequenced, and based on one single nucleotide polymorphism(SNP) site unique to Angelica sinensis, a nucleotide signature consisting of 37-bp (5'-aatccgcgtc atcttagtga gctcaaggac ccttagg-3') was developed. It is highly conserved and specific within Angelica sinensis while divergent among other species. Then, we designed primers (DG01F/DG01R) to amplify the nucleotide signature region from processed materials. 15 samples procured online were analysed. By seeking the signature, we found that 7 of them were counterfeits. 28 batches of Chinese patent medicines containing Danggui were amplified. 19 of them were found to contain the signature, and adulterants such as Ligusticum sinense, Notopterygium incisum, Angelica decursiva and Angelica gigas were detected in other batches. Thus, this nucleotide signature, with only 37-bp, will broaden the application of DNA barcoding to identify the components in decoctions, Chinese patent medicines and other products with degraded DNA.
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