ArticlePloS one2016
A Comparison of the Costs and Benefits of Bacterial Gene Expression.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Bacterial chromosomal gene positioning is likely shaped by selection on both mean and growth-dependent expression.PLoS genetics · 2026Article
- Non-canonical resource allocation in heterotrophically growing Thermoanaerobacter kivui.Nature communications · 2025Article
- A mismatch between slow protein synthesis and fast environmental fluctuations determines tradeoffs in bacterial proteome allocation strategies.bioRxiv : the preprint server for biology · 2025Article
- Investigating the role of theFEMS microbes · 2025Article
- CRP improves the survival and competitive fitness ofJournal of bacteriology · 2024Article
- Metabolic Profile of the Genome-ReducedACS synthetic biology · 2024Article
- The Landscape and Perspectives of the Human Gut Metaproteomics.Molecular & cellular proteomics : MCP · 2024Article
- Whole genome-scale assessment of gene fitness of Novosphingobium aromaticavorans during spaceflight.BMC genomics · 2023Article
- Evaluating E. coli genome-scale metabolic model accuracy with high-throughput mutant fitness data.Molecular systems biology · 2023Article
- Three Novel Antisense Overlapping Genes in E. coli O157:H7 EDL933.Microbiology spectrum · 2023Article
- Group A streptococci induce stronger M protein-fibronectin interaction when specific human antibodies are bound.Frontiers in microbiology · 2023Article
- Article
- Redefining fundamental concepts of transcription initiation in bacteria.Nature reviews. Genetics · 2020Review
- The size of the immune repertoire of bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Modeling the multi-scale mechanisms of macromolecular resource allocation.Current opinion in microbiology · 2018Review
- Evolutionary Plasticity of AmrZ Regulation inmSphere · 2018Article
- Article
- Cellular trade-offs and optimal resource allocation during cyanobacterial diurnal growth.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Principles of proteome allocation are revealed using proteomic data and genome-scale models.Scientific reports · 2016Article
- A Theoretical Lower Bound for Selection on the Expression Levels of Proteins.Genome biology and evolution · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To study how a bacterium allocates its resources, we compared the costs and benefits of most (86%) of the proteins in Escherichia coli K-12 during growth in minimal glucose medium. The cost or investment in each protein was estimated from ribosomal profiling data, and the benefit of each protein was measured by assaying a library of transposon mutants. We found that proteins that are important for fitness are usually highly expressed, and 95% of these proteins are expressed at above 13 parts per million (ppm). Conversely, proteins that do not measurably benefit the host (with a benefit of less than 5% per generation) tend to be weakly expressed, with a median expression of 13 ppm. In aggregate, genes with no detectable benefit account for 31% of protein production, or about 22% if we correct for genetic redundancy. Although some of the apparently unnecessary expression could have subtle benefits in minimal glucose medium, the majority of the burden is due to genes that are important in other conditions. We propose that at least 13% of the cell's protein is "on standby" in case conditions change.
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