ArticlePloS one2016
De novo Analysis of the Epiphytic Transcriptome of the Cucurbit Powdery Mildew Fungus Podosphaera xanthii and Identification of Candidate Secreted Effector Proteins.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Transcriptomic Analysis Insights into Salt Adaptation of Pickle-DerivedMicroorganisms · 2026Article
- Transcriptome sequence resource for the hazelnut powdery mildew pathogen Erysiphe corylacearum.Scientific data · 2026Article
- The mutant STAY-GREEN (Molecular breeding : new strategies in plant improvement · 2024Article
- A fungal CFEM-containing effector targets NPR1 regulator NIMIN2 to suppress plant immunity.Plant biotechnology journal · 2024Article
- Systematic identification and functional characterization of the CFEM proteins in fishscale bamboo rhombic-spot pathogenFrontiers in plant science · 2024Article
- Suppression of Chitin-Triggered Immunity by Plant Fungal Pathogens: A Case Study of the Cucurbit Powdery Mildew FungusJournal of fungi (Basel, Switzerland) · 2023Review
- Suppression of Chitin-Triggered Immunity by a New Fungal Chitin-Binding Effector Resulting from Alternative Splicing of a Chitin Deacetylase Gene.Journal of fungi (Basel, Switzerland) · 2022Article
- Gene Mining for Conserved, Non-Annotated Proteins ofJournal of fungi (Basel, Switzerland) · 2021Article
- The Role of Glycoside Hydrolases in Phytopathogenic Fungi and Oomycetes Virulence.International journal of molecular sciences · 2021Review
- A haustorial-expressed lytic polysaccharide monooxygenase from the cucurbit powdery mildew pathogen Podosphaera xanthii contributes to the suppression of chitin-triggered immunity.Molecular plant pathology · 2021Article
- Elucidation of resistance signaling and identification of powdery mildew resistant mapping loci (ClaPMR2) during watermelon-Podosphaera xanthii interaction using RNA-Seq and whole-genome resequencing approach.Scientific reports · 2020Article
- Genome-Wide Identification of Effector Candidates With Conserved Motifs From the Wheat Leaf Rust FungusFrontiers in microbiology · 2020Article
- Effector mining from the Erysiphe pisi haustorial transcriptome identifies novel candidates involved in pea powdery mildew pathogenesis.Molecular plant pathology · 2019Article
- The haustorial transcriptome of the cucurbit pathogen Podosphaera xanthii reveals new insights into the biotrophy and pathogenesis of powdery mildew fungi.BMC genomics · 2019Article
- Transcriptome sequence resource for the cucurbit powdery mildew pathogen Podosphaera xanthii.Scientific data · 2019Article
- RNA-seq analysis and fluorescence imaging of melon powdery mildew disease reveal an orchestrated reprogramming of host physiology.Scientific reports · 2019Article
- TransFlow: a modular framework for assembling and assessing accurate de novo transcriptomes in non-model organisms.BMC bioinformatics · 2018Article
- Transformation by growth onto agro-infiltrated tissues (TGAT), a simple and efficient alternative for transient transformation of the cucurbit powdery mildew pathogen Podosphaera xanthii.Molecular plant pathology · 2018Article
- Transcriptome and miRNA analyses of the response to Corynespora cassiicola in cucumber.Scientific reports · 2018Article
- BcCFEM1, a CFEM Domain-Containing Protein with Putative GPI-Anchored Site, Is Involved in Pathogenicity, Conidial Production, and Stress Tolerance inFrontiers in microbiology · 2017Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
The cucurbit powdery mildew fungus Podosphaera xanthii is a major limiting factor for cucurbit production worldwide. Despite the fungus's agronomic and economic importance, very little is known about fundamental aspects of P. xanthii biology, such as obligate biotrophy or pathogenesis. To design more durable control strategies, genomic information about P. xanthii is needed. Powdery mildews are fungal pathogens with large genomes compared with those of other fungi, which contain vast amounts of repetitive DNA sequences, much of which is composed of retrotransposons. To reduce genome complexity, in this work we aimed to obtain and analyse the epiphytic transcriptome of P. xanthii as a starting point for genomic research. Total RNA was isolated from epiphytic fungal material, and the corresponding cDNA library was sequenced using a 454 GS FLX platform. Over 676,562 reads were obtained and assembled into 37,241 contigs. Annotation data identified 8,798 putative genes with different orthologues. As described for other powdery mildew fungi, a similar set of missing core ascomycete genes was found, which may explain obligate biotrophy. To gain insight into the plant-pathogen relationships, special attention was focused on the analysis of the secretome. After this analysis, 137 putative secreted proteins were identified, including 53 candidate secreted effector proteins (CSEPs). Consistent with a putative role in pathogenesis, the expression profile observed for some of these CSEPs showed expression maxima at the beginning of the infection process at 24 h after inoculation, when the primary appressoria are mostly formed. Our data mark the onset of genomics research into this very important pathogen of cucurbits and shed some light on the intimate relationship between this pathogen and its host plant.
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