ArticleNature protocols2016
Remodeling somatic nuclei via exogenous expression of protamine 1 to create spermatid-like structures for somatic nuclear transfer.
Article in Nature protocols, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 32 citations in OpenAlex.
- Generation of modified cows and sheep from spermatid-like haploid embryonic stem cells.Nature biotechnology · 2026Article
- Genome-edited farm animals from haploid stem cells.Nature biotechnology · 2026Article
- The challenges and prospects of mammalian reproduction in extraterrestrial environments.The Journal of reproduction and development · 2026Review
- Article
- Can Humanity Thrive Beyond the Galaxy?The Journal of reproduction and development · 2025Review
- SPAG17 mediates nuclear translocation of protamines during spermiogenesis.Frontiers in cell and developmental biology · 2023Article
- Somatic Cell Nuclear Transfer Using Freeze-Dried Protaminized Donor Nuclei.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Embryo biotechnologies in sheep: Achievements and new improvements.Reproduction in domestic animals = Zuchthygiene · 2022Review
- Sperm bauplan and function and underlying processes of sperm formation and selection.Physiological reviews · 2022Review
- The expression, function, and utilization of Protamine1: a literature review.Translational cancer research · 2021Review
- Animal cloning and consumption of its by-products: A scientific and Islamic perspectives.Saudi journal of biological sciences · 2021Review
- Insights into the roles of sperm in animal cloning.Stem cell research & therapy · 2020Review
- Nuclear quiescence and histone hyper-acetylation jointly improve protamine-mediated nuclear remodeling in sheep fibroblasts.PloS one · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This protocol describes how to convert the chromatin structure of sheep and mouse somatic cells into spermatid-like nuclei through the heterologous expression of the protamine 1 gene (Prm1). Furthermore, we also provide step-by-step instructions for somatic cell nuclear transfer (SCNT) of Prm1-remodeled somatic nuclei in sheep oocytes. There is evidence that changing the organization of a somatic cell nucleus with that which mirrors the spermatozoon nucleus leads to better nuclear reprogramming. The protocol may have further potential application in determining the protamine and histone footprints of the whole genome; obtaining 'gametes' from somatic cells; and furthering understanding of the molecular mechanisms regulating the maintenance of DNA methylation in imprinted control regions during male gametogenesis. The protocol is straightforward, and it requires 4 weeks from the establishment of the cell lines to their transfection and the production of cloned blastocysts. It is necessary for researchers to have experience in cell biology and embryology, with basic skills in molecular biology, to carry out the protocol.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.