ArticleNature protocols2016
Serial sequencing of isolength RAD tags for cost-efficient genome-wide profiling of genetic and epigenetic variations.
Article in Nature protocols, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Article
- Genomic Insights into Genetic Diversity of Coilia nasus Across Wild Ecotypes and Farmed Populations in China.Marine biotechnology (New York, N.Y.) · 2026Article
- Holo-2bRAD: A Hologenomic Method for High-Resolution Analysis of Coral Microbiomes During Bleaching.Microorganisms · 2026Article
- The microbiome of interstitial cystitis revealed by 2bRAD-M.Scientific reports · 2026Article
- 2b-RAD sequencing of Malus florentina populations reveals strong population structure and signals of balancing selection at disease resistance loci.BMC plant biology · 2025Article
- Supragingival Plaque Microbial Signatures Between Radiation Related Caries and Common Caries.International dental journal · 2025Article
- The Intertidal North-South Split: Oceanographic Features and Life History Shape the Phylogeography of ChitonEvolutionary applications · 2025Article
- Development of Single-Nucleotide Polymorphism (SNP)-Based Species-Specific Real-Time PCR Assays for Authenticating Five Highly Priced Tuna.Foods (Basel, Switzerland) · 2024Article
- Tracking the hologenome dynamics in aquatic invertebrates by the holo-2bRAD approach.Communications biology · 2024Article
- Analysis of Elimination Effects of Inbreeding on Genotype Frequency in Larval Stages of Chinese Shrimp.Biology · 2024Article
- Signatures of selection inEvolutionary applications · 2024Article
- Oral microbiota disorder in GC patients revealed by 2b-RAD-M.Journal of translational medicine · 2023Article
- Genetic Diversity and Population Structure ofBiology · 2023Article
- Screening and characterization of sex-specific sequences through 2b-RAD sequencing in American shad (Alosa sapidissima).PloS one · 2023Article
- The bladder microbiome of NMIBC and MIBC patients revealed by 2bRAD-M.Frontiers in cellular and infection microbiology · 2023Article
- Comparative phylogeography of two commensal rat species (Ecology and evolution · 2022Article
- The renal pelvis urobiome in the unilateral kidney stone patients revealed by 2bRAD-M.Journal of translational medicine · 2022Article
- Integrated Methylome and Transcriptome Analysis Provides Insights into the DNA Methylation Underlying the Mechanism of Cytoplasmic Male Sterility in Kenaf (International journal of molecular sciences · 2022Article
- Genetic Diversity and Genetic Differentiation of Populations of Golden-Backed Carp (Animals : an open access journal from MDPI · 2022Article
- IIb-RAD-sequencing coupled with random forest classification indicates regional population structuring and sex-specific differentiation in salmon lice (Ecology and evolution · 2022Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Isolength restriction site-associated DNA (isoRAD) sequencing is a very simple but powerful approach that was originally developed for genome-wide genotyping at minimal labor and cost, and it has recently extended its applicability to allow quantification of DNA methylation levels. The isoRAD method is distinct from other genotyping-by-sequencing (GBS) methods because of its use of special restriction enzymes to produce isolength tags (32-36 bp), and sequencing of these uniform tags can bring many benefits. However, the relatively short tags produced by the original protocol are mostly suited to single-end (SE) sequencing (36-50 bp), and therefore they cannot efficiently match the gradually increased sequencing capacity of next-generation sequencing (NGS) platforms. To address this issue, we describe an advanced protocol that allows the preparation of five concatenated isoRAD tags for Illumina paired-end (PE) sequencing (100-150 bp). The configuration of the five concatenated tags is highly flexible, and can be defined by users to work with a desired combination of samples and/or restriction enzymes to suit specific research purposes. In comparison with the original protocol, the advanced protocol has an additional digestion and ligation step, and library preparation can be completed in ∼8 h.
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