ArticleJournal of biomolecular NMR2016
A simple and robust protocol for high-yield expression of perdeuterated proteins in Escherichia coli grown in shaker flasks.
Article in Journal of biomolecular NMR, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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Who cites it
32 citing papers in PubMed.
- Tangled Up in Fibers: How a Multidomain Lytic Polysaccharide Monooxygenase Binds Its Chitin Substrate.ACS applied materials & interfaces · 2026Article
- M9 medium composition alters E. coli metabolism during recombinant expression.Journal of biomolecular NMR · 2026Article
- Signal-strapping as a protein-sequence search method for the discovery of metalloproteins.Nature communications · 2025Article
- A flexible, allosteric loop regulates protein activity and rewires electrostatics.Protein science : a publication of the Protein Society · 2025Article
- Dynamic networks connect the USP14 active site region with the proteasome interaction surface.Protein science : a publication of the Protein Society · 2025Article
- An NMR sample preparation case study: Considerations for the self-destructive protease caspase-6.PloS one · 2025Article
- Labeling of methyl groups: a streamlined protocol and guidance for the selection ofJournal of biomolecular NMR · 2024Article
- A Transient, Excited Species of the Autoinhibited α-State of the Bacterial Transcription Factor RfaH May Represent an Early Intermediate on the Fold-Switching Pathway.Biochemistry · 2024Article
- Article
- UsingBiochemistry · 2024Article
- Human cystatin C induces the disaggregation process of selected amyloid beta peptides: a structural and kinetic view.Scientific reports · 2023Article
- UsingbioRxiv : the preprint server for biology · 2023Article
- Mixed, nonclassical behavior in a classic allosteric protein.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Perdeuterated GbpA Enables Neutron Scattering Experiments of a Lytic Polysaccharide Monooxygenase.ACS omega · 2023Article
- Promiscuity of response regulators for thioredoxin steers bacterial virulence.Nature communications · 2022Article
- Solid-State NMRThe journal of physical chemistry. A · 2022Article
- Many dissimilar NusG protein domains switch between α-helix and β-sheet folds.Nature communications · 2022Article
- Current status of neutron crystallography in structural biology.Biophysics and physicobiology · 2022Article
- Visualizing an Allosteric Intermediate Using CuAAC Stabilization of an NMR Mixed Labeled Dimer.ACS chemical biology · 2021Article
- Solution NMR backbone assignments of the N-terminal Zα-linker-Zβ segment from Homo sapiens ADAR1p150.Biomolecular NMR assignments · 2021Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
We present a simple, convenient and robust protocol for expressing perdeuterated proteins in E. coli BL21(DE3) cells in shaker flasks that reduces D
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.