ArticleScientific reports2016
Developing a genetic manipulation system for the Antarctic archaeon, Halorubrum lacusprofundi: investigating acetamidase gene function.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 23 citations in OpenAlex.
- Distinct Patterns of Antibiotic Sensitivities in Ammonia-Oxidising Archaea.Environmental microbiology · 2025Article
- Haloarchaea as Promising Chassis to Green Chemistry.Microorganisms · 2024Review
- The parasitic lifestyle of an archaeal symbiont.Nature communications · 2024Article
- Improving the genetic system forFrontiers in microbiology · 2023Article
- Cas1 and Fen1 Display Equivalent Functions During Archaeal DNA Repair.Frontiers in microbiology · 2022Article
- Evolution of Protein Structure and Stability in Global Warming.International journal of molecular sciences · 2020Review
- Interaction of two strongly divergent archaellins stabilizes the structure of the Halorubrum archaellum.MicrobiologyOpen · 2020Article
- Division plane placement in pleomorphic archaea is dynamically coupled to cell shape.Molecular microbiology · 2019Article
- Unexpected host dependency of Antarctic Nanohaloarchaeota.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Recent advances in understanding extremophiles.F1000Research · 2019Review
- Genomic variation and biogeography of Antarctic haloarchaea.Microbiome · 2018Article
- A plasmid from an Antarctic haloarchaeon uses specialized membrane vesicles to disseminate and infect plasmid-free cells.Nature microbiology · 2017Article
- Morphological and proteomic analysis of biofilms from the Antarctic archaeon, Halorubrum lacusprofundi.Scientific reports · 2016Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
No systems have been reported for genetic manipulation of cold-adapted Archaea. Halorubrum lacusprofundi is an important member of Deep Lake, Antarctica (~10% of the population), and is amendable to laboratory cultivation. Here we report the development of a shuttle-vector and targeted gene-knockout system for this species. To investigate the function of acetamidase/formamidase genes, a class of genes not experimentally studied in Archaea, the acetamidase gene, amd3, was disrupted. The wild-type grew on acetamide as a sole source of carbon and nitrogen, but the mutant did not. Acetamidase/formamidase genes were found to form three distinct clades within a broad distribution of Archaea and Bacteria. Genes were present within lineages characterized by aerobic growth in low nutrient environments (e.g. haloarchaea, Starkeya) but absent from lineages containing anaerobes or facultative anaerobes (e.g. methanogens, Epsilonproteobacteria) or parasites of animals and plants (e.g. Chlamydiae). While acetamide is not a well characterized natural substrate, the build-up of plastic pollutants in the environment provides a potential source of introduced acetamide. In view of the extent and pattern of distribution of acetamidase/formamidase sequences within Archaea and Bacteria, we speculate that acetamide from plastics may promote the selection of amd/fmd genes in an increasing number of environmental microorganisms.
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