ArticleScientific reports2016
Humic acid inhibits HBV-induced autophagosome formation and induces apoptosis in HBV-transfected Hep G2 cells.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- Fecal Microbiota Transplantation in Hepatitis B e Antigen-Positive Chronic Hepatitis B Patients: A Pilot Study.Digestive diseases and sciences · 2021Trial
- Metabolic associated fatty liver disease induced hepatocyte apoptosis to eliminate hepatitis B virus.Virology journal · 2026Article
- Pharmacological Effects of Humic Substances and the Signaling Mechanisms of Their Implementation.Bulletin of experimental biology and medicine · 2026Review
- Biomedical Applications of Humic Substances: From Natural Biopolymers to Therapeutic Agents.Antioxidants (Basel, Switzerland) · 2025Review
- Assessing the safety of thermal mineral water for cosmetic applications: an integrated approach using physicochemical, cheminformatics, and bioinformatics techniques.RSC advances · 2025Article
- Inhibition of hepatitis B virusWorld journal of gastroenterology · 2024Article
- The role of Traditional Chinese medicine in anti-HBV: background, progress, and challenges.Chinese medicine · 2023Review
- Activation of apoptosis and G0/G1 cell cycle arrest along with inhibition of melanogenesis by humic acid and fulvic acid:Iranian journal of basic medical sciences · 2022Article
- Clinical review of humic acid as an antiviral: Leadup to translational applications in clinical humeomics.Frontiers in pharmacology · 2022Review
- Neddylation inhibitor MLN4924 has anti-HBV activity via modulating the ERK-HNF1α-C/EBPα-HNF4α axis.Journal of cellular and molecular medicine · 2021Article
- Mummy Induces Apoptosis Through Inhibiting of Epithelial-Mesenchymal Transition (EMT) in Human Breast Cancer Cells.Galen medical journal · 2020Article
- S100A4 promotes lung tumor development through β-catenin pathway-mediated autophagy inhibition.Cell death & disease · 2018Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hepatitis B Virus (HBV) utilizes several mechanisms to survive in the host cells and one of the main pathways being autophagosome formation. Humic acid (HA), one of the major components of Mineral pitch, is an Ayurvedic medicinal food, commonly used by the people of the Himalayan regions of Nepal and India for various body ailments. We hypothesized that HA could induce cell death and inhibit HBV-induced autophagy in hepatic cells. Incubation of Hep G2.2.1.5 cells (HepG2 cells stably expressing HBV) with HA (100 μM) inhibited both cell proliferation and autophagosome formation significantly, while apoptosis induction was enhanced. Western blot results showed that HA incubation resulted in decreased levels of beclin-1, SIRT-1 and c-myc, while caspase-3 and β-catenin expression were up-regulated. Western blot results showed that HA significantly inhibited the expression of HBx (3-fold with 50 μM and 5-fold with 100 μM) compared to control cells. When HA was incubated with HBx-transfected Hep G2 cells, HBx-induced autophagosome formation and beclin-1 levels were decreased. These data showed that HA induced apoptosis and inhibited HBV-induced autophagosome formation and proliferation in hepatoma cells.
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