ArticleNature communications2016
A modular platform for one-step assembly of multi-component membrane systems by fusion of charged proteoliposomes.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Membrane-Associated Biomolecules for Synthetic Cell Signalling.Chembiochem : a European journal of chemical biology · 2026Review
- Advances in Strategies for Colloidal Self-Assembly.Chemical reviews · 2026Review
- Spatially regulated mRNA translation enables functional membrane protein integration in synthetic cells.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Towards a perfusion system for functional study of membrane proteins with independent control of the electrical and chemical transmembrane potential.Biophysical reviews · 2025Review
- Modulating Liposome Surface Charge for Maximized ATP Regeneration in Synthetic Nanovesicles.ACS synthetic biology · 2024Article
- SecYEG-mediated translocation in a model synthetic cell.Synthetic biology (Oxford, England) · 2024Article
- Function Investigations and Applications of Membrane Proteins on Artificial Lipid Membranes.International journal of molecular sciences · 2023Review
- Experimental platform for the functional investigation of membrane proteins in giant unilamellar vesicles.Soft matter · 2022Article
- Increased efficiency of charge-mediated fusion in polymer/lipid hybrid membranes.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A Microfluidic Platform for Sequential Assembly and Separation of Synthetic Cell Models.ACS synthetic biology · 2021Article
- Single Cell-like Systems Reveal Active Unidirectional and Light-Controlled Transport by Nanomachineries.ACS nano · 2021Article
- Rapid functionalisation and detection of viruses via a novel CaScientific reports · 2019Article
- Design and Applications of a Fluorescent Labeling Technique for Lipid and Surfactant Preformed Vesicles.ACS omega · 2019Article
- Electrophysiological measurement of ion channels on plasma/organelle membranes using an on-chip lipid bilayer system.Scientific reports · 2018Article
- Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.Journal of visualized experiments : JoVE · 2018Article
- Cryo-EM structures of the autoinhibitedeLife · 2016Article
- Building protein networks in synthetic systems from the bottom-up.Biotechnology advancesReview
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Authors and funding
3 authors.
Funding
Abstract
An important goal in synthetic biology is the assembly of biomimetic cell-like structures, which combine multiple biological components in synthetic lipid vesicles. A key limiting assembly step is the incorporation of membrane proteins into the lipid bilayer of the vesicles. Here we present a simple method for delivery of membrane proteins into a lipid bilayer within 5 min. Fusogenic proteoliposomes, containing charged lipids and membrane proteins, fuse with oppositely charged bilayers, with no requirement for detergent or fusion-promoting proteins, and deliver large, fragile membrane protein complexes into the target bilayers. We demonstrate the feasibility of our method by assembling a minimal electron transport chain capable of adenosine triphosphate (ATP) synthesis, combining Escherichia coli F
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