ArticleMolecular biology of the cell2016
madSTORM: a superresolution technique for large-scale multiplexing at single-molecule accuracy.
Article in Molecular biology of the cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Multi-channel automated programmable liquid exchange system enables high-quality sample preparation for super-resolution microscopy.Innovation (Cambridge (Mass.)) · 2026Article
- Photonic waveguide chip-based nanoscopy visualizes rearrangements of the cortical actin cytoskeleton in activated Jurkat T cells.Science advances · 2026Article
- Automated Cyclic Super-Resolution Microscopy for Nanoscale Protein Mapping.bioRxiv : the preprint server for biology · 2026Article
- Multiplexed Nanoscopy via Buffer Exchange.ACS nano · 2024Article
- Heterogeneity of Signaling Complex Nanostructure in T Cells Activated Via the T Cell Antigen Receptor.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2023Article
- Article
- Affimers and nanobodies as molecular probes and their applications in imaging.Journal of cell science · 2022Article
- Understanding immune signaling using advanced imaging techniques.Biochemical Society transactions · 2022Review
- Novel Tools to Measure Single Molecules Colocalization in Fluorescence Nanoscopy by Image Cross Correlation Spectroscopy.Nanomaterials (Basel, Switzerland) · 2022Article
- Challenges facing quantitative large-scale optical super-resolution, and some simple solutions.iScience · 2021Review
- Surface Modification of Fluorescent Nanodiamonds for Biological Applications.Nanomaterials (Basel, Switzerland) · 2021Review
- The Decade of Super-Resolution Microscopy of the Presynapse.Frontiers in synaptic neuroscience · 2020Review
- A Picture Worth a Thousand Molecules-Integrative Technologies for Mapping Subcellular Molecular Organization and Plasticity in Developing Circuits.Frontiers in synaptic neuroscience · 2020Article
- Microclusters as T Cell Signaling Hubs: Structure, Kinetics, and Regulation.Frontiers in cell and developmental biology · 2020Review
- A Perspective on Fluorescent Nanodiamond Bioimaging.Small (Weinheim an der Bergstrasse, Germany) · 2019Review
- Differential nanoscale organisation of LFA-1 modulates T-cell migration.Journal of cell science · 2019Article
- Brilliant blue, green, yellow, and red fluorescent diamond particles: synthesis, characterization, and multiplex imaging demonstrations.Nanoscale · 2019Article
- Registration and Visualization of Correlative Super-Resolution Microscopy Data.Biophysical journal · 2019Article
- Review Article: Synthesis, properties, and applications of fluorescent diamond particles.Journal of vacuum science and technology. B, Nanotechnology & microelectronics : materials, processing, measurement, & phenomena : JVST B · 2019Review
- Targeting Fluorescent Nanodiamonds to Vascular Endothelial Growth Factor Receptors in Tumor.Bioconjugate chemistry · 2019Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Investigation of heterogeneous cellular structures using single-molecule localization microscopy has been limited by poorly defined localization accuracy and inadequate multiplexing capacity. Using fluorescent nanodiamonds as fiducial markers, we define and achieve localization precision required for single-molecule accuracy in dSTORM images. Coupled with this advance, our new multiplexing strategy, madSTORM, allows accurate targeting of multiple molecules using sequential binding and elution of fluorescent antibodies. madSTORM is used on an activated T-cell to localize 25 epitopes, 14 of which are on components of the same multimolecular T-cell receptor complex. We obtain an average localization precision of 2.6 nm, alignment error of 2.0 nm, and <0.01% cross-talk. Combining these technical advances affords the ability to move beyond obtaining superresolved structures to defining spatial relationships among constituent molecules within structures. Probing the molecular topology of complex signaling cascades and other heterogeneous networks is feasible with madSTORM.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.