ArticleMolecular biology of the cell2016
An NXF1 mRNA with a retained intron is expressed in hippocampal and neocortical neurons and is translated into a protein that functions as an Nxf1 cofactor.
Article in Molecular biology of the cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 36 citations in OpenAlex.
- Article
- Exitrons: offering new roles to retained introns-the novel regulators of protein diversity and utility.AoB PLANTS · 2024Review
- Measurement of HIV Rev-Rev Response Element Functional Activity.Methods in molecular biology (Clifton, N.J.) · 2024Article
- RNA in axons, dendrites, synapses and beyond.Frontiers in molecular neuroscience · 2024Review
- Replicative aging in yeast involves dynamic intron retention patterns associated with mRNA processing/export and protein ubiquitination.Microbial cell (Graz, Austria) · 2024Article
- Retroviral RNA Processing.Viruses · 2022Review
- Article
- Differential fates of introns in gene expression due to global alternative splicing.Human genetics · 2022Review
- Selective Modulation of a Pan-Essential Protein as a Therapeutic Strategy in Cancer.Cancer discovery · 2021Article
- Evolution and diversification of the nuclear pore complex.Biochemical Society transactions · 2021Review
- CRISPR/Cas9 Genome Editing of the Human Topoisomerase IIMolecular pharmacology · 2021Article
- Spatiotemporal Regulation of Transcript Isoform Expression in the Hippocampus.Frontiers in molecular neuroscience · 2021Article
- Sequence and Functional Variation in the HIV-1 Rev Regulatory Axis.Current HIV research · 2020Article
- Challenges in detecting and quantifying intron retention from next generation sequencing data.Computational and structural biotechnology journal · 2020Review
- Effects of DNA topoisomerase IIα splice variants on acquired drug resistance.Cancer drug resistance (Alhambra, Calif.) · 2020Article
- A novel retroviral vector system to analyze expression from mRNA with retained introns using fluorescent proteins and flow cytometry.Scientific reports · 2019Article
- Escaping nuclear decay: the significance of mRNA export for gene expression.Current genetics · 2019Review
- Nonsense-mediated RNA decay in the brain: emerging modulator of neural development and disease.Nature reviews. Neuroscience · 2018Review
- An important class of intron retention events in human erythroblasts is regulated by cryptic exons proposed to function as splicing decoys.RNA (New York, N.Y.) · 2018Article
- SRSF3 promotes pluripotency througheLife · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The Nxf1 protein is a major nuclear export receptor for the transport of mRNA, and it also is essential for export of retroviral mRNAs with retained introns. In the latter case, it binds to RNA elements known as constitutive transport elements (CTEs) and functions in conjunction with a cofactor known as Nxt1. The NXF1 gene also regulates expression of its own intron-containing RNA through the use of a functional CTE within intron 10. mRNA containing this intron is exported to the cytoplasm, where it can be translated into the 356-amino acid short Nxf1(sNxf1) protein, despite the fact that it is a prime candidate for nonsense-mediated decay (NMD). Here we demonstrate that sNxf1 is highly expressed in nuclei and dendrites of hippocampal and neocortical neurons in rodent brain. Additionally, we show that sNxf1 localizes in RNA granules in neurites of differentiated N2a mouse neuroblastoma cells, where it shows partial colocalization with Staufen2 isoform SS, a protein known to play a role in dendritic mRNA trafficking. We also show that sNxf1 forms heterodimers in conjunction with the full-length Nxf1 and that sNxf1 can replace Nxt1 to enhance the expression of CTE-containing mRNA and promote its association with polyribosomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.