ArticleScience (New York, N.Y.)2016
A global genetic interaction network maps a wiring diagram of cellular function.
Open access · greenAbstract read
In one paragraph
Article in Science (New York, N.Y.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 775 papers.
0numbers the graph read from it
0cells of the map it votes in
775citing papers in PubMed
80.2field-weighted citation impact, top 1% of its field
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
775 citing papers in PubMed, 1,370 citations in OpenAlex.
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715 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
54 authors at 8 institutions in 5 countries.
Michael CostanzoThe Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
Benjamin VanderSluisDepartment of Computer Science and Engineering, University of Minnesota-Twin Cities, 200 Union Street, Minneapolis, MN 55455, USA. Simons Center for Data Analysis, Simons Foundation, 160 Fifth Avenue, New York, NY 10010, USA.
Elizabeth N KochDepartment of Computer Science and Engineering, University of Minnesota-Twin Cities, 200 Union Street, Minneapolis, MN 55455, USA.
Anastasia BaryshnikovaLewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA.
Carles PonsDepartment of Computer Science and Engineering, University of Minnesota-Twin Cities, 200 Union Street, Minneapolis, MN 55455, USA.
Guihong TanThe Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
Wen WangDepartment of Computer Science and Engineering, University of Minnesota-Twin Cities, 200 Union Street, Minneapolis, MN 55455, USA.
Matej UsajThe Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
Julia HanchardThe Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1. Department of Molecular Genetics, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
Susan D LeeDepartment of Developmental, Molecular and Chemical Biology, Tufts University School of Medicine, Boston, MA 02111, USA.
Vicent PelechanoEuropean Molecular Biology Laboratory (EMBL), Genome Biology Unit, 69117 Heidelberg, Germany.
Erin B StylesThe Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1. Department of Molecular Genetics, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
Maximilian BillmannDivision of Signaling and Functional Genomics, German Cancer Research Center (DKFZ) and Heidelberg University, Heidelberg, Germany.
Jolanda van LeeuwenThe Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
Nydia van DykThe Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
Zhen-Yuan LinLunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto ON, Canada.
Elena KuzminThe Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1. Department of Molecular Genetics, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
Justin NelsonDepartment of Computer Science and Engineering, University of Minnesota-Twin Cities, 200 Union Street, Minneapolis, MN 55455, USA. Program in Biomedical Informatics and Computational Biology, University of Minnesota-Twin Cities, 200 Union Street, Minneapolis, MN 55455, USA.
Jeff S PiotrowskiThe Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1. Chemical Genomics Research Group, RIKEN Center for Sustainable Resource Sciences (CSRS), Saitama, Japan.
Tharan SrikumarPrincess Margaret Cancer Centre, University Health Network and Department of Medical Biophysics, University of Toronto, Toronto ON, Canada.
Sondra BahrThe Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
Yiqun ChenThe Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
Raamesh DeshpandeDepartment of Computer Science and Engineering, University of Minnesota-Twin Cities, 200 Union Street, Minneapolis, MN 55455, USA.
Christoph F KuratThe Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
Sheena C LiThe Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1. Chemical Genomics Research Group, RIKEN Center for Sustainable Resource Sciences (CSRS), Saitama, Japan.
Zhijian LiThe Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
Mojca Mattiazzi UsajThe Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
Hiroki OkadaDepartment of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba, Japan 277-8561.
Natasha PascoeThe Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1. Department of Molecular Genetics, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
Bryan-Joseph San LuisThe Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
Sara SharifpoorThe Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
Emira ShuteriqiThe Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
Scott W SimpkinsDepartment of Computer Science and Engineering, University of Minnesota-Twin Cities, 200 Union Street, Minneapolis, MN 55455, USA. Program in Biomedical Informatics and Computational Biology, University of Minnesota-Twin Cities, 200 Union Street, Minneapolis, MN 55455, USA.
Jamie SniderThe Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
Harsha Garadi SureshThe Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
Yizhao TanThe Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
Hongwei ZhuThe Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
Noel Malod-DogninComputer Science Deptartment, University College London, London WC1E 6BT, UK.
Vuk JanjicDepartment of Computing, Imperial College London, UK.
Natasa PrzuljComputer Science Deptartment, University College London, London WC1E 6BT, UK. School of Computing (RAF), Union University, Belgrade, Serbia.
Olga G TroyanskayaSimons Center for Data Analysis, Simons Foundation, 160 Fifth Avenue, New York, NY 10010, USA. Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA.
Igor StagljarThe Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1. Department of Molecular Genetics, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1. Department of Biochemistry, University of Toronto, Toronto, ON, Canada.
Tian XiaDepartment of Computer Science and Engineering, University of Minnesota-Twin Cities, 200 Union Street, Minneapolis, MN 55455, USA. School of Electronic Information and Communications, Huazhong University of Science and Technology, Wuhan, China, 430074.
Yoshikazu OhyaDepartment of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba, Japan 277-8561.
Anne-Claude GingrasDepartment of Molecular Genetics, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1. Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto ON, Canada.
Brian RaughtPrincess Margaret Cancer Centre, University Health Network and Department of Medical Biophysics, University of Toronto, Toronto ON, Canada.
Michael BoutrosDivision of Signaling and Functional Genomics, German Cancer Research Center (DKFZ) and Heidelberg University, Heidelberg, Germany.
Lars M SteinmetzEuropean Molecular Biology Laboratory (EMBL), Genome Biology Unit, 69117 Heidelberg, Germany. Department of Genetics, School of Medicine and Stanford Genome Technology Center Stanford University, Palo Alto, CA 94304, USA.
Claire L MooreDepartment of Developmental, Molecular and Chemical Biology, Tufts University School of Medicine, Boston, MA 02111, USA.
Adam P RosebrockThe Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1. Department of Molecular Genetics, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
Amy A CaudyThe Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1. Department of Molecular Genetics, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
Chad L MyersDepartment of Computer Science and Engineering, University of Minnesota-Twin Cities, 200 Union Street, Minneapolis, MN 55455, USA. Program in Biomedical Informatics and Computational Biology, University of Minnesota-Twin Cities, 200 Union Street, Minneapolis, MN 55455, USA. cmyers@cs.umn.edu brenda.andrews@utoronto.ca charlie.boone@utoronto.ca.
Brenda AndrewsThe Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1. Department of Molecular Genetics, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1. cmyers@cs.umn.edu brenda.andrews@utoronto.ca charlie.boone@utoronto.ca.
Charles BooneThe Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1. Department of Molecular Genetics, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1. Chemical Genomics Research Group, RIKEN Center for Sustainable Resource Sciences (CSRS), Saitama, Japan. cmyers@cs.umn.edu brenda.andrews@utoronto.ca charlie.boone@utoronto.ca.
University of Minnesota · USEuropean Molecular Biology Laboratory · DEHeidelberg University · DEPrinceton University · USTufts University · USHuazhong University of Science and Technology · CNImperial College London · GBUniversity of Toronto · CA
Funding
Mapping the reference genetic network of a eukaryotic cellR01HG005853 · NHGRI · UNIVERSITY OF TORONTO · PI ANDREWS, BRENDA JEAN, BOONE, CHARLES · 2010 to 2025
$7.6MMethods for large-scale analysis of chemical-genetic interactionsR01HG005084 · NHGRI · UNIVERSITY OF MINNESOTA · PI MYERS, CHAD L · 2010 to 2022
$2.9MSparse Structural Equation Models for Gene Networks and Chemical GenomicsR01GM104975 · NIGMS · UNIVERSITY OF MIAMI CORAL GABLES · PI CAI, XIAODONG · 2012 to 2016
$1.8MCIHR FDN-143264CIHR FDN-143265European Research Council 294542NHGRI NIH HHS R01 HG005084NHGRI NIH HHS R01 HG005853NIGMS NIH HHS R01 GM104975
Abstract
We generated a global genetic interaction network for Saccharomyces cerevisiae, constructing more than 23 million double mutants, identifying about 550,000 negative and about 350,000 positive genetic interactions. This comprehensive network maps genetic interactions for essential gene pairs, highlighting essential genes as densely connected hubs. Genetic interaction profiles enabled assembly of a hierarchical model of cell function, including modules corresponding to protein complexes and pathways, biological processes, and cellular compartments. Negative interactions connected functionally related genes, mapped core bioprocesses, and identified pleiotropic genes, whereas positive interactions often mapped general regulatory connections among gene pairs, rather than shared functionality. The global network illustrates how coherent sets of genetic interactions connect protein complex and pathway modules to map a functional wiring diagram of the cell.
Indexed as
Gene Regulatory NetworksEpistasis, GeneticGenes, EssentialGenes, FungalGenetic PleiotropySaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSaccharomyces cerevisiae Proteins
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.