Evidence map›Paper›PMID 27707939›Full record

ArticleJournal of clinical microbiology2016

Comparative Genomic Analysis of Haemophilus haemolyticus and Nontypeable Haemophilus influenzae and a New Testing Scheme for Their Discrimination.

Fang Hu, Lavanya Rishishwar, Ambily Sivadas, Gabriel J Mitchell, I King Jordan, Timothy F Murphy, Janet R Gilsdorf, Leonard W Mayer, Xin Wang

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 11 citations in OpenAlex.

  1. Trial
  2. Article
  3. Article
  4. Article
  5. Comparative Genomics ofFrontiers in veterinary science · 2021
    Article
  6. Draft Genome Sequences for a Diverse Set of SevenMicrobiology resource announcements · 2018
    Article
  7. Article
  8. Review
  9. Whole-genome analysis of haemophilus influenzae invasive strains isolated from Campinas state University hospital. An epidemiological approach 2012 - 2019 and ancestor strains.The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 3 countries.

Fang HuMeningitis and Vaccine Preventable Diseases Branch, Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Lavanya RishishwarSchool of Biology, Georgia Institute of Technology, Atlanta, Georgia, USA.
Ambily SivadasSchool of Biology, Georgia Institute of Technology, Atlanta, Georgia, USA.
Gabriel J MitchellSchool of Biology, Georgia Institute of Technology, Atlanta, Georgia, USA.
I King JordanSchool of Biology, Georgia Institute of Technology, Atlanta, Georgia, USA.
Timothy F MurphyDivision of Infectious Diseases, Department of Medicine, Clinical and Translational Research Center, Department of Microbiology and Immunology, University at Buffalo, State University of New York, Buffalo, New York, USA.
Janet R GilsdorfDepartment of Pediatrics, University of Michigan, Ann Arbor, Michigan, USA.
Leonard W MayerMeningitis and Vaccine Preventable Diseases Branch, Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Xin WangMeningitis and Vaccine Preventable Diseases Branch, Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA gqe8@cdc.gov.
Georgia Institute of Technology · USNational Center for Immunization and Respiratory Diseases · USCenters for Disease Control and Prevention · USUniversity at Buffalo, State University of New York · USUniversity of Michigan–Ann Arbor · US

Funding

University of Buffalo Clinical and Translational Science Institute - Supplement SchulyerUL1TR001412 · NCATS · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI MURPHY, TIMOTHY F · 2015 to 2024
$33.8M
Persistent H. influenzae in COPD - virulence, vaccines and antibiotic resistanceR01AI019641 · NIAID · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI MURPHY, TIMOTHY F · 1985 to 2023
$7.9M
NCATS NIH HHS UL1 TR001412NIAID NIH HHS R01 AI019641
6 · The paper itself

Abstract

Haemophilus haemolyticus has been recently discovered to have the potential to cause invasive disease. It is closely related to nontypeable Haemophilus influenzae (NT H. influenzae). NT H. influenzae and H. haemolyticus are often misidentified because none of the existing tests targeting the known phenotypes of H. haemolyticus are able to specifically identify H. haemolyticus Through comparative genomic analysis of H. haemolyticus and NT H. influenzae, we identified genes unique to H. haemolyticus that can be used as targets for the identification of H. haemolyticus A real-time PCR targeting purT (encoding phosphoribosylglycinamide formyltransferase 2 in the purine synthesis pathway) was developed and evaluated. The lower limit of detection was 40 genomes/PCR; the sensitivity and specificity in detecting H. haemolyticus were 98.9% and 97%, respectively. To improve the discrimination of H. haemolyticus and NT H. influenzae, a testing scheme combining two targets (H. haemolyticus purT and H. influenzae hpd, encoding protein D lipoprotein) was also evaluated and showed 96.7% sensitivity and 98.2% specificity for the identification of H. haemolyticus and 92.8% sensitivity and 100% specificity for the identification of H. influenzae, respectively. The dual-target testing scheme can be used for the diagnosis and surveillance of infection and disease caused by H. haemolyticus and NT H. influenzae.

Indexed as

Base SequenceComparative Genomic HybridizationDNA, BacterialGenome, BacterialHaemophilus InfectionsHaemophilus influenzaeHumansLimit of DetectionLipoproteinsPhosphoribosylglycinamide FormyltransferaseReal-Time Polymerase Chain ReactionSensitivity and SpecificitySequence Analysis, DNADNA, BacterialLipoproteinsPhosphoribosylglycinamide Formyltransferase

Identifiers

PMID27707939
PMCPMC5121393
OpenAlexW2529073199

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.