ArticleGenetics2016
The Identification of a Novel Mutant Allele of topoisomerase II in Caenorhabditis elegans Reveals a Unique Role in Chromosome Segregation During Spermatogenesis.
Article in Genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 40 citations in OpenAlex.
- Article
- Loss of meiotic double strand breaks triggers recruitment of recombination-independent pro-crossover factors in C. elegans spermatogenesis.PLoS genetics · 2025Article
- The TOP-2/condensin II axis silences transcription during germline specification in C. elegans.G3 (Bethesda, Md.) · 2024Article
- Article
- Nutrient Deficiencies Impact on the Cellular and Metabolic Responses of Saxitoxin ProducingMarine drugs · 2023Article
- Extensive Bioinformatics Analyses Reveal a Phylogenetically Conserved Winged Helix (WH) Domain (Zτ) of Topoisomerase IIα, Elucidating Its Very High Affinity for Left-Handed Z-DNA and Suggesting Novel Putative Functions.International journal of molecular sciences · 2023Article
- Article
- Measuring Embryonic Viability and Brood Size in Caenorhabditis elegans.Journal of visualized experiments : JoVE · 2023Article
- Topoisomerases I and II facilitate condensin DC translocation to organize and repress X chromosomes in C. elegans.Molecular cell · 2022Article
- Article
- DNA repair, recombination, and damage signaling.Genetics · 2022Review
- Endogenous localization of TOP-2 inmicroPublication biology · 2021Article
- Harnessing the power of genetics: fast forward genetics in Caenorhabditis elegans.Molecular genetics and genomics : MGG · 2021Review
- A Role forCells · 2020Article
- Topoisomerases Modulate the Timing of Meiotic DNA Breakage and Chromosome Morphogenesis inGenetics · 2020Article
- Identification of Suppressors ofG3 (Bethesda, Md.) · 2020Article
- GCNA Preserves Genome Integrity and Fertility Across Species.Developmental cell · 2020Article
- GCNA Interacts with Spartan and Topoisomerase II to Regulate Genome Stability.Developmental cell · 2020Article
- The demethylase NMAD-1 regulates DNA replication and repair in the Caenorhabditis elegans germline.PLoS genetics · 2019Article
- A Genomic Region ContainingG3 (Bethesda, Md.) · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Topoisomerase II alleviates DNA entanglements that are generated during mitotic DNA replication, transcription, and sister chromatid separation. In contrast to mitosis, meiosis has two rounds of chromosome segregation following one round of DNA replication. In meiosis II, sister chromatids segregate from each other, similar to mitosis. Meiosis I, on the other hand, segregates homologs, which requires pairing, synapsis, and recombination. The exact role that topoisomerase II plays during meiosis is unknown. In a screen reexamining Caenorhabditis elegans legacy mutants isolated 30 years ago, we identified a novel allele of the gene encoding topoisomerase II, top-2(it7). In this study, we demonstrate that top-2(it7) males produce dead embryos, even when fertilizing wild-type oocytes. Characterization of early embryonic events indicates that fertilization is successful and sperm components are transmitted to the embryo. However, sperm chromatin is not detected in these fertilized embryos. Examination of top-2(it7) spermatogenic germ lines reveals that the sperm DNA fails to segregate properly during anaphase I of meiosis, resulting in anucleate sperm. top-2(it7) chromosome-segregation defects observed during anaphase I are not due to residual entanglements incurred during meiotic DNA replication and are not dependent on SPO-11-induced double-strand DNA breaks. Finally, we show that TOP-2 associates with chromosomes in meiotic prophase and that chromosome association is disrupted in the germ lines of top-2(it7) mutants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.