ArticleAnnals of botany2017
Gene conversion events and variable degree of homogenization of rDNA loci in cultivars of Brassica napus.
Article in Annals of botany, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- Dynamic evolution and hierarchical regulation of rDNA in polyploid oat.The Plant journal : for cell and molecular biology · 2026Article
- Nuclear Ribosomal ITS1-5.8S rDNA-ITS2 Region as a Phylogenetic Marker forInternational journal of molecular sciences · 2026Article
- Article
- Does time matter? Intraspecific diversity of ribosomal RNA genes in lineages of the allopolyploid model grass Brachypodium hybridum with different evolutionary ages.BMC plant biology · 2024Article
- Defining autopolyploidy: Cytology, genetics, and taxonomy.American journal of botany · 2024Review
- Intragenomic rDNA variation - the product of concerted evolution, mutation, or something in between?Heredity · 2023Review
- Homoeologous non-reciprocal translocation explains a major QTL for seed lignin content in oilseed rape (Brassica napus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Uniparental expression of ribosomal RNA in ×Frontiers in plant science · 2023Article
- The Chromosome Number and rDNA Loci Evolution inInternational journal of molecular sciences · 2022Article
- Tracing the Evolution of the Angiosperm Genome from the Cytogenetic Point of View.Plants (Basel, Switzerland) · 2022Review
- The Nuclear 35S rDNA World in Plant Systematics and Evolution: A Primer of Cautions and Common Misconceptions in Cytogenetic Studies.Frontiers in plant science · 2022Review
- To Be or Not to Be Expressed: The First Evidence of a Nucleolar Dominance Tissue-Specificity inFrontiers in plant science · 2021Article
- Chromosome instabilities in resynthesized Brassica napus revealed by FISH.Journal of applied genetics · 2020Article
- The fate of 35S rRNA genes in the allotetraploid grass Brachypodium hybridum.The Plant journal : for cell and molecular biology · 2020Article
- Comparative Chromosomal Localization of 45S and 5S rDNA Sites in 76 Purple-Fleshed Sweet Potato Cultivars.Plants (Basel, Switzerland) · 2020Article
- Genome Size Variation and Comparative Genomics Reveal Intraspecific Diversity inFrontiers in plant science · 2020Article
- Hexaploid sweetpotato (Ipomoea batatas (L.) Lam.) may not be a true type to either auto- or allopolyploid.PloS one · 2020Article
- Intragenomic heterogeneity of intergenic ribosomal DNA spacers in Cucurbita moschata is determined by DNA minisatellites with variable potential to form non-canonical DNA conformations.DNA research : an international journal for rapid publication of reports on genes and genomes · 2019Article
- Molecular cytogenetic characterisation of Elytrigia ×mucronata, a natural hybrid of E. intermedia and E. repens (Triticeae, Poaceae).BMC plant biology · 2019Article
- Ribosomal DNA loci derived from Brachypodium stacei are switched off for major parts of the life cycle of Brachypodium hybridum.Journal of experimental botany · 2019Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsBrassica napus (AACC, 2n = 38, oilseed rape) is a relatively recent allotetraploid species derived from the putative progenitor diploid species Brassica rapa (AA, 2n = 20) and Brassica oleracea (CC, 2n = 18). To determine the influence of intensive breeding conditions on the evolution of its genome, we analysed structure and copy number of rDNA in 21 cultivars of B. napus, representative of genetic diversity.
methodsWe used next-generation sequencing genomic approaches, Southern blot hybridization, expression analysis and fluorescence in situ hybridization (FISH). Subgenome-specific sequences derived from rDNA intergenic spacers (IGS) were used as probes for identification of loci composition on chromosomes. KEY
resultsMost B. napus cultivars (18/21, 86 %) had more A-genome than C-genome rDNA copies. Three cultivars analysed by FISH ('Darmor', 'Yudal' and 'Asparagus kale') harboured the same number (12 per diploid set) of loci. In B. napus 'Darmor', the A-genome-specific rDNA probe hybridized to all 12 rDNA loci (eight on the A-genome and four on the C-genome) while the C-genome-specific probe showed weak signals on the C-genome loci only. Deep sequencing revealed high homogeneity of arrays suggesting that the C-genome genes were largely overwritten by the A-genome variants in B. napus 'Darmor'. In contrast, B. napus 'Yudal' showed a lack of gene conversion evidenced by additive inheritance of progenitor rDNA variants and highly localized hybridization signals of subgenome-specific probes on chromosomes. Brassica napus 'Asparagus kale' showed an intermediate pattern to 'Darmor' and 'Yudal'. At the expression level, most cultivars (95 %) exhibited stable A-genome nucleolar dominance while one cultivar ('Norin 9') showed co-dominance.
conclusionsThe B. napus cultivars differ in the degree and direction of rDNA homogenization. The prevalent direction of gene conversion (towards the A-genome) correlates with the direction of expression dominance indicating that gene activity may be needed for interlocus gene conversion.
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