ArticlePloS one2016
Identification of Unanticipated and Novel N-Acyl L-Homoserine Lactones (AHLs) Using a Sensitive Non-Targeted LC-MS/MS Method.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 69 citations in OpenAlex.
- Pseudomonas aeruginosa Quorum Signals; Associations with Virulence, and Impact of Therapeutic Gamma Radiation Doses.Current microbiology · 2025Article
- Emerging advances in biosensor technologies for quorum sensing signal molecules.Analytical and bioanalytical chemistry · 2025Review
- Structural characterization of N-acyl-homoserine lactones from bacterial quorum sensing using LC-MS/MS analyses after Paternò-Büchi derivatization in solution.Analytical and bioanalytical chemistry · 2024Article
- Development of an analytical method to quantify N-acyl-homoserine lactones in bacterial cultures, river water, and treated wastewater.Analytical and bioanalytical chemistry · 2024Article
- Metabolic insights from mass spectrometry imaging of biofilms: A perspective from model microorganisms.Methods (San Diego, Calif.) · 2024Review
- AmBio · 2023Article
- Quorum sensing activities and genomic insights of plant growth-promoting rhizobacteria isolated from Assam tea.World journal of microbiology & biotechnology · 2023Article
- Unusual enantiomeric D,L-N-acyl homoserine lactones in Pectobacterium atrosepticum and Pseudomonas aeruginosa.PloS one · 2023Article
- Subcellular redox responses reveal different Cu-dependent antioxidant defenses between mitochondria and cytosol.Metallomics : integrated biometal science · 2022Article
- Label-Free Electrochemical Aptasensor for the Detection of the 3-O-CBiosensors · 2022Article
- Microevolution of theFrontiers in microbiology · 2022Article
- Bacterial Lighthouses-Real-Time Detection ofBiosensors · 2021Article
- Metabolomic profiling of Burkholderia cenocepacia in synthetic cystic fibrosis sputum medium reveals nutrient environment-specific production of virulence factors.Scientific reports · 2021Article
- Methylotroph Quorum Sensing Signal Identification by Inverse Stable Isotopic Labeling.ACS chemical biology · 2021Article
- Identification of a solo acylhomoserine lactone synthase from the myxobacterium Archangium gephyra.Scientific reports · 2021Article
- Targeted and untargeted quantification of quorum sensing signalling molecules in bacterial cultures and biological samples via HPLC-TQ MS techniques.Analytical and bioanalytical chemistry · 2021Article
- Bioactive Natural Products in Actinobacteria Isolated in Rainwater From Storm Clouds Transported by Western Winds in Spain.Frontiers in microbiology · 2021Article
- Diversity of Acyl Homoserine Lactone Molecules in Anaerobic Membrane Bioreactors Treating Sewage at Psychrophilic Temperatures.Membranes · 2020Article
- Liquid Crystal Emulsions That Intercept and Report on Bacterial Quorum Sensing.ACS applied materials & interfaces · 2020Article
- Bacterial Quorum Sensing Signals Self-Assemble in Aqueous Media to Form Micelles and Vesicles: An Integrated Experimental and Molecular Dynamics Study.The journal of physical chemistry. B · 2020Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
N-acyl L-homoserine lactones (AHLs) constitute a predominant class of quorum-sensing signaling molecules used by Gram-negative bacteria. Here, we report a sensitive and non-targeted HPLC-MS/MS method based on parallel reaction monitoring (PRM) to identify and quantitate known, unanticipated, and novel AHLs in microbial samples. Using a hybrid quadrupole-high resolution mass analyzer, this method integrates MS scans and all-ion fragmentation MS/MS scans to allow simultaneous detection of AHL parent-ion masses and generation of full mass spectra at high resolution and high mass accuracy in a single chromatographic run. We applied this method to screen for AHL production in a variety of Gram-negative bacteria (i.e. B. cepacia, E. tarda, E. carotovora, E. herbicola, P. stewartii, P. aeruginosa, P. aureofaciens, and R. sphaeroides) and discovered that nearly all of them produce a larger set of AHLs than previously reported. Furthermore, we identified production of an uncommon AHL (i.e. 3-oxo-C7-HL) in E. carotovora and P. stewartii, whose production has only been previously observed within the genera Serratia and Yersinia. Finally, we used our method to quantitate AHL degradation in B. cepacia, E. carotovora, E. herbicola, P. stewartii, P. aeruginosa, P. aureofaciens, the non-AHL producer E. coli, and the Gram-positive bacterium B. subtilis. We found that AHL degradation ability varies widely across these microbes, of which B. subtilis and E. carotovora are the best degraders, and observed that there is a general trend for AHLs containing long acyl chains (≥10 carbons) to be degraded at faster rates than AHLs with short acyl chains (≤6 carbons).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.