Evidence map›Paper›PMID 27706188›Full record

ArticlePloS one2016

Edible Safety Assessment of Genetically Modified Rice T1C-1 for Sprague Dawley Rats through Horizontal Gene Transfer, Allergenicity and Intestinal Microbiota.

Kai Zhao, Fangfang Ren, Fangting Han, Qiwen Liu, Guogan Wu, Yan Xu, Jian Zhang, Xiao Wu, Jinbin Wang, Peng Li and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
3.4field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 5 institutions in 2 countries.

Kai ZhaoBiotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, People's Republic of China.
Fangfang RenCollege of Life and Environment Sciences, Shanghai Normal University, Shanghai, People's Republic of China.
Fangting HanCollege of Life and Environment Sciences, Shanghai Normal University, Shanghai, People's Republic of China.
Qiwen LiuCollege of Life and Environment Sciences, Shanghai Normal University, Shanghai, People's Republic of China.
Guogan WuBiotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, People's Republic of China.
Yan XuCollege of Life and Environment Sciences, Shanghai Normal University, Shanghai, People's Republic of China.
Jian ZhangAlberta Innovates-Technology Futures, Vegreville, Alberta, Canada.
Xiao WuBiotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, People's Republic of China.
Jinbin WangBiotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, People's Republic of China.
Peng LiBiotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, People's Republic of China.
Wei ShiCollege of Life and Environment Sciences, Shanghai Normal University, Shanghai, People's Republic of China.
Hong ZhuBiotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, People's Republic of China.
Jianjun LvNational Center for Safety Evaluation of Drugs, Beijing Economic-Technological Development Area, Beijing, People's Republic of China.
Xiao ZhaoLife Science College, Ningxia University, Yinchuan, Ningxia, People's Republic of China.
Xueming TangBiotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, People's Republic of China.
Shanghai Normal University · CNBiotechnology Research Institute · CNShanghai Academy of Agricultural Sciences · CNAlberta Innovates · CANingxia University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study, assessment of the safety of transgenic rice T1C-1 expressing Cry1C was carried out by: (1) studying horizontal gene transfer (HGT) in Sprague Dawley rats fed transgenic rice for 90 d; (2) examining the effect of Cry1C protein in vitro on digestibility and allergenicity; and (3) studying the changes of intestinal microbiota in rats fed with transgenic rice T1C-1 in acute and subchronic toxicity tests. Sprague Dawley rats were fed a diet containing either 60% GM Bacillus thuringiensis (Bt) rice T1C-1 expressing Cry1C protein, the parental rice Minghui 63, or a basic diet for 90 d. The GM Bt rice T1C-1 showed no evidence of HGT between rats and transgenic rice. Sequence searching of the Cry1C protein showed no homology with known allergens or toxins. Cry1C protein was rapidly degraded in vitro with simulated gastric and intestinal fluids. The expressed Cry1C protein did not induce high levels of specific IgG and IgE antibodies in rats. The intestinal microbiota of rats fed T1C-1 was also analyzed in acute and subchronic toxicity tests by DGGE. Cluster analysis of DGGE profiles revealed significant individual differences in the rats' intestinal microbiota.

Indexed as

AllergensAnimalsBacillus thuringiensisBacteriaBacterial ProteinsCluster AnalysisFecesFemaleFood, Genetically ModifiedGenetic VariationGene Transfer, HorizontalImmunoglobulin GImmunoglobulin MInsect ProteinsIntestinal MucosaIntestinesAllergensBacterial ProteinsCry toxin receptorsImmunoglobulin GImmunoglobulin MInsect ProteinsReceptors, Cell SurfaceRNA, Ribosomal, 16S

Identifiers

PMID27706188
PMCPMC5051820
OpenAlexW2529510388

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.