ArticleBiophysical journal2016
Measuring the Viscosity of the Escherichia coli Plasma Membrane Using Molecular Rotors.
Article in Biophysical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 102 citations in OpenAlex.
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- Toxic Effects of Butanol in the Plane of the Cell Membrane.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Lipid Rafts in Signalling, Diseases, and Infections: What Can Be Learned from Fluorescence Techniques?Membranes · 2025Review
- Factors affecting biofilm formation by bacteria on fabrics.International microbiology : the official journal of the Spanish Society for Microbiology · 2024Article
- Visualizing Actin Packing and the Effects of Actin Attachment on Lipid Membrane Viscosity Using Molecular Rotors.JACS Au · 2024Article
- Molecular Rotors: Fluorescent Sensors for Microviscosity and Conformation of Biomolecules.Angewandte Chemie (International ed. in English) · 2024Review
- A Mildly Acidic Environment Alters Pseudomonas aeruginosa Virulence and Causes Remodeling of the Bacterial Surface.Microbiology spectrum · 2023Article
- Viscosity-Sensitive Membrane Dyes as Tools To Estimate the Crystalline Structure of Lipid Bilayers.Analytical chemistry · 2023Article
- Bacterial lipid biophysics and membrane organization.Current opinion in microbiology · 2023Review
- Directly imaging emergence of phase separation in peroxidized lipid membranes.Communications chemistry · 2023Article
- Efficacy of acidified water-in-oil emulsions against desiccatedFrontiers in microbiology · 2023Article
- Probing Liquid-Ordered and Disordered Phases in Lipid Model Membranes: A Combined Theoretical and Spectroscopic Study of a Fluorescent Molecular Rotor.The journal of physical chemistry. B · 2022Article
- Investigation of apoptosis based on fluorescence lifetime imaging microscopy with a mitochondria-targeted viscosity probe.RSC advances · 2021Article
- Rigidochromism by imide functionalisation of an aminomaleimide fluorophore.Chemical science · 2021Article
- Imaging non-classical mechanical responses of lipid membranes using molecular rotors.Chemical science · 2020Article
- Bacterial Vivisection: How Fluorescence-Based Imaging Techniques Shed a Light on the Inner Workings of Bacteria.Microbiology and molecular biology reviews : MMBR · 2020Review
- Light-Driven ATP Regeneration in Diblock/Grafted Hybrid Vesicles.Chembiochem : a European journal of chemical biology · 2020Article
- Role of the lipid bilayer in outer membrane protein folding in Gram-negative bacteria.The Journal of biological chemistry · 2020Review
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The viscosity is a highly important parameter within the cell membrane, affecting the diffusion of small molecules and, hence, controlling the rates of intracellular reactions. There is significant interest in the direct, quantitative assessment of membrane viscosity. Here we report the use of fluorescence lifetime imaging microscopy of the molecular rotor BODIPY C10 in the membranes of live Escherichia coli bacteria to permit direct quantification of the viscosity. Using this approach, we investigated the viscosity in live E. coli cells, spheroplasts, and liposomes made from E. coli membrane extracts. For live cells and spheroplasts, the viscosity was measured at both room temperature (23°C) and the E. coli growth temperature (37°C), while the membrane extract liposomes were studied over a range of measurement temperatures (5-40°C). At 37°C, we recorded a membrane viscosity in live E. coli cells of 950 cP, which is considerably higher than that previously observed in other live cell membranes (e.g., eukaryotic cells, membranes of Bacillus vegetative cells). Interestingly, this indicates that E. coli cells exhibit a high degree of lipid ordering within their liquid-phase plasma membranes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.