ArticleBiophysical journal2016
Modulating Vesicle Adhesion by Electric Fields.
Article in Biophysical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 35 citations in OpenAlex.
- Dynamic Shape Modulation of Deflated and Adhered Lipid Vesicles.Journal of the American Chemical Society · 2025Article
- Electrochemical Droplet Sculpturing of Short Carbon Fiber Nanotip Electrodes for Neurotransmitter Detection.ACS electrochemistry · 2025Article
- Rolling vesicles: From confined rotational flows to surface-enabled motion.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Assessing membrane material properties from the response of giant unilamellar vesicles to electric fields.Advances in physics: X · 2023Article
- Investigation of Shape Transformations of Vesicles, Induced by Their Adhesion to Flat Substrates Characterized by Different Adhesion Strength.International journal of molecular sciences · 2021Article
- Morphologies of Vesicle Doublets: Competition among Bending Elasticity, Surface Tension, and Adhesion.Biophysical journal · 2020Article
- The GDP-Bound State of Mitochondrial Mfn1 Induces Membrane Adhesion of Apposing Lipid Vesicles through a Cooperative Binding Mechanism.Biomolecules · 2020Article
- Observations of Membrane Domain Reorganization in Mechanically Compressed Artificial Cells.Chembiochem : a European journal of chemical biology · 2019Article
- Glycan-decorated protocells: novel features for rebuilding cellular processes.Interface focus · 2019Review
- Adhesion of Active Cytoskeletal Vesicles.Biophysical journal · 2018Article
- Supramolecular zippers elicit interbilayer adhesion of membranes producing cell death.Biochimica et biophysica acta. General subjects · 2018Article
- Charged giant unilamellar vesicles prepared by electroformation exhibit nanotubes and transbilayer lipid asymmetry.Scientific reports · 2018Article
- Lectin-mediated protocell crosslinking to mimic cell-cell junctions and adhesion.Scientific reports · 2018Article
- Clathrin polymerization exhibits high mechano-geometric sensitivity.Soft matter · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We introduce an experimental setup for modulating adhesion of giant unilamellar vesicles to a planar substrate. Adhesion is induced by the application of an external potential to a transparent indium tin oxide-coated electrode (the substrate), which enables single-vesicle studies. We demonstrate tunable and reversible adhesion of negatively charged vesicles. The adhesion energy at different potentials is calculated from the vesicle shape assessed with confocal microscopy. Two approaches for these estimates are employed: one based on the whole contour of the vesicle and a second based on the contact curvature of the membrane in the vicinity of the substrate. Both approaches agree well with each other and show that the adhering vesicles are in the weak adhesion regime for the range of explored external potentials. Using fluorescence quenching assays, we detect that, in the adhering membrane segment, only the outer bilayer leaflet of the vesicle is depleted of negatively charged fluorescent lipids, while the inner leaflet remains unaffected. We show that depletion of negatively charged lipids is consistent Poisson-Boltzmann theory, taking into account charge regulation from lipid mobility. Finally, we also show that lipid diffusion is not significantly affected in the adhering membrane segment. We believe that the approaches introduced here for modulating and assessing vesicle adhesion have many potential applications in the field of single-vesicle studies and research on membrane adhesion.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.