Evidence map›Paper›PMID 27704689›Full record

ArticleHaemophilia : the official journal of the World Federation of Hemophilia2016

Connective tissue growth factor (CTGF/CCN2) in haemophilic arthropathy and arthrofibrosis: a histological analysis.

J Jiang, N L Leong, U Khalique, T M Phan, K M Lyons, J V Luck

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Haemophilia : the official journal of the World Federation of Hemophilia, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed, 1 pooled it
0.3field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 1 synthesis or guideline pooled it, 11 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Review
  4. Article
  5. Hemophilic arthropathy: Current knowledge and future perspectives.Journal of thrombosis and haemostasis : JTH · 2021
    Review
  6. Gasotransmitters: Potential Therapeutic Molecules of Fibrotic Diseases.Oxidative medicine and cellular longevity · 2021
    Review
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

J JiangHemophilia Treatment Center at Orthopaedic Institute for Children, Los Angeles, CA, USA.
N L LeongDepartment of Orthopaedic Surgery, David Geffen School of Medicine at University of California, Los Angeles, CA, USA.
U KhaliqueDepartment of Orthopaedic Surgery, David Geffen School of Medicine at University of California, Los Angeles, CA, USA.
T M PhanDepartment of Orthopaedic Surgery, David Geffen School of Medicine at University of California, Los Angeles, CA, USA.
K M LyonsDepartment of Orthopaedic Surgery, David Geffen School of Medicine at University of California, Los Angeles, CA, USA.
J V LuckHemophilia Treatment Center at Orthopaedic Institute for Children, Los Angeles, CA, USA.
University of California, Los Angeles · USOrthopaedic Institute for Children · US

Funding

MARC U*STAR Program at the University of California, Los AngelesT34GM008563 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI MCEVOY, MEGAN M, POE, GINA R · 1996 to 2023
$9.7M
UCLA MSD ProgramR25GM055052 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BARBER, PAUL HENRY, HASSON, TAMA W. · 1996 to 2021
$9.2M
CCN PROTEINS IN CARTILAGE FORMATION AND MAINTENANCER01AR052686 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI LYONS, KAREN M. · 2005 to 2015
$3.9M
NIAMS NIH HHS R01 AR052686NIGMS NIH HHS R25 GM055052NIGMS NIH HHS T34 GM008563
6 · The paper itself

Abstract

introductionJoint haemorrhage is the principal clinical manifestation of haemophilia frequently leading to advanced arthropathy and arthrofibrosis, resulting in severe disability. The degree and prevalence of arthrofibrosis in hemophilic arthropathy is more severe than in other forms of arthropathy. Expression of connective tissue growth factor (CTGF) has been linked to many fibrotic diseases, but has not been studied in the context of haemophilic arthropathy.

aimWe aim to compare synovial tissues histologically from haemophilia and osteoarthritis patients with advanced arthropathy in order to compare expression of proteins that are possibly aetiologic in the development of arthrofibrosis.

methodsHuman synovial tissues were obtained from 10 haemophilia and 10 osteoarthritis patients undergoing joint surgery and processed for histology and immunohistochemistry.

resultsAll samples from haemophilia patients had synovitis with hypertrophy and hyperplasia of synovial villi. Histologically, synovial tissues contained hyperplastic villi with increased cellularity and abundant haemosiderin- and ferritin-pigmented macrophage-like cells (HMCs), with a perivascular localization in the sub-surface layer. CTGF staining was observed in the surface layer and sub-surface layer in all haemophilia patients, exclusively co-localizing with HMCs. Quantification showed that the extent of CTGF-positive areas was correlated with the degree of detection of HMCs. CTGF was not observed in any of the samples from osteoarthritis patients.

conclusionUsing histological analysis, we showed that CTGF expression is elevated in haemophilia patients with arthrofibrosis and absent in patients with osteoarthritis. Additionally, we found that CTGF is always associated with haemosiderin-pigmented macrophage-like cells, which suggests that CTGF is produced by synovial A cells following the uptake of blood breakdown products.

Indexed as

AdultConnective Tissue Growth FactorFemaleHemarthrosisHemophilia AHumansJoint DiseasesMaleMiddle AgedYoung AdultCCN2 protein, humanConnective Tissue Growth FactorarthrofibrosisCTGFhaemophilic arthropathyhaemosiderinmacrophagessynovium

Identifiers

PMID27704689
PMCPMC5166983
OpenAlexW2529155458

What OpenQuestion holds

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LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.