ReviewHistochemistry and cell biology2016
Deep tissue imaging: a review from a preclinical cancer research perspective.
Review in Histochemistry and cell biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 64 citations in OpenAlex.
- First application of three-dimensional light sheet fluorescence microscopy to human testicular tumors: New perspectives in histopathology.Andrology · 2025Article
- Collagen crosslinking-induced corneal morphological changes: a three-dimensional light sheet Microscopy-based evaluation.Scientific reports · 2024Article
- Frontiers in artificial intelligence-directed light-sheet microscopy for uncovering biological phenomena and multi-organ imaging.View (Beijing, China) · 2024Article
- Bridging Smart Nanosystems with Clinically Relevant Models and Advanced Imaging for Precision Drug Delivery.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Applications of multiphoton microscopy in imaging cerebral and retinal organoids.Frontiers in neuroscience · 2024Review
- Tissue clearing and immunostaining to visualize the spatial organization of vasculature and tumor cells in mouse liver.Frontiers in oncology · 2023Article
- ROCKETS - a novel one-for-all toolbox for light sheet microscopy in drug discovery.Frontiers in immunology · 2023Article
- Design and application of organic contrast agents for molecular imaging in the second near infrared (NIR-II) window.Photoacoustics · 2022Review
- Optical tissue clearing associated with 3D imaging: application in preclinical and clinical studies.Histochemistry and cell biology · 2022Review
- In focus in HCB.Histochemistry and cell biology · 2022Article
- A Novel Three-Dimensional Imaging System Based on Polysaccharide Staining for Accurate Histopathological Diagnosis of Inflammatory Bowel Diseases.Cellular and molecular gastroenterology and hepatology · 2022Article
- Tissue clearing and 3D imaging in developmental biology.Development (Cambridge, England) · 2021Review
- Tissue clearing technique: Recent progress and biomedical applications.Journal of anatomy · 2021Review
- An advanced optical clearing protocol allows label-free detection of tissue necrosisTheranostics · 2021Article
- Article
- Influence ofInternational journal of molecular sciences · 2020Article
- Light sheet fluorescence microscopy guided MALDI-imaging mass spectrometry of cleared tissue samples.Scientific reports · 2020Article
- Biomedical Applications of Tissue Clearing and Three-Dimensional Imaging in Health and Disease.iScience · 2020Review
- Lanthanide-Based Optical Probes of Biological Systems.Cell chemical biology · 2020Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This review delves into the rapidly evolving field of deep tissue imaging at cellular resolution, reviewing popular tissue clearing and staining methods in combination with light-sheet fluorescence microscopy (LSFM) including quantification and three-dimensional visualization tools, the field of applications and perspective, particularly with the focus on preclinical cancer research and drug development. The LSFM technique presented here allows an extremely fast optical sectioning for three-dimensional reconstruction of centimeter-sized tissue samples at cellular resolution. However, optical clearing methods are required to receive optical transparent tissue. Application of either tissue autofluorescence, in vivo fluorescence labeling, endogenous fluorescence or ex vivo whole-mount immunolabeling enables three-dimensional in situ visualization of morphological and functional features of unsectioned whole-mount tissue samples. This powerful and innovative imaging technique opens up a new dimension of tissue analysis providing detailed and comprehensive insights into biology. It enables the investigation of normal and pathological tissue features and disease progression and allows precise monitoring of potential therapeutic interventions in intact biological tissue on a cellular level.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.