ArticleScience advances2016
Depth-resolved mid-infrared photothermal imaging of living cells and organisms with submicrometer spatial resolution.
Article in Science advances, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 164 papers.
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Who cites it
164 citing papers in PubMed.
- Demystifying Experimental Parameters in Optical Photothermal Imaging in Aqueous Media.ACS omega · 2026Article
- Chem-SIM: SIM-resolved chemical imaging via wide-field mid-infrared photothermal modulation of fluorescence.Nature communications · 2026Article
- Biological and Biomedical Applications of Optical Photothermal Infrared Spectroscopy (O-PTIR).Applied spectroscopy · 2026Review
- Structural, Compositional, and Dielectric State Profiling in Label-Free Single-Cell Monitoring.Small methods · 2026Review
- Single-Particle Mid-Infrared Photothermal Imaging Reveals Hidden Heterogeneity in Real-World Micro- and Nanoplastics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Stimulated Raman photothermal microscopy: theory and implementation.Advances in optics and photonics · 2026Article
- Fix or freeze? Spectral differences arising from tissue preparation in chemical imaging.The Analyst · 2026Article
- Improved Microplastic Identification from Simultaneously Collected Photothermal Infrared and Raman Spectra Using Multiview Conformal Prediction.ACS measurement science au · 2026Article
- FILM: mapping organellar metabolism by mid-infrared photothermal-modulated fluorescence.Nature methods · 2026Article
- The Next Frontier in Vibrational Imaging: Chemically Designed Raman Probes Pass the Spectral Barrier.JACS Au · 2026Review
- FILM: Mapping organellar metabolism by mid-infrared photothermal modulated fluorescence.ArXiv · 2026Article
- Single-Cell Metabolic Imaging and Digital Scoring of Fat Tissue Remodeling by Label-Free Metabolic Microscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Extracting and characterizing microplastics and nanoplastics from environmental samples.Nature protocols · 2026Review
- Nanoscale mapping of composition and orientation in electrospun polymeric nanofibers loaded with carbon atomic wires.Scientific reports · 2026Article
- Far-field single-molecule vibrational spectroscopy and imaging.Chemical science · 2026Review
- IR-AMES uncovers structure and composition of Alzheimer's tau oligomers.bioRxiv : the preprint server for biology · 2026Article
- Revealing hidden tissue architecture with mid-infrared dichroism photoacoustic microscopy.Light, science & applications · 2026Article
- The Serendipitous Rise of Mid-infrared Photothermal Microscopy.Chemical & biomedical imaging · 2026Article
- Mid-infrared microscopy for label-free digital staining: initial clinical assessment.Frontiers in bioengineering and biotechnology · 2026Article
- Fix or Freeze? Spectral Differences Arising from Tissue Preparation in Chemical Imaging.bioRxiv : the preprint server for biology · 2025Article
104 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chemical contrast has long been sought for label-free visualization of biomolecules and materials in complex living systems. Although infrared spectroscopic imaging has come a long way in this direction, it is thus far only applicable to dried tissues because of the strong infrared absorption by water. It also suffers from low spatial resolution due to long wavelengths and lacks optical sectioning capabilities. We overcome these limitations through sensing vibrational absorption-induced photothermal effect by a visible laser beam. Our mid-infrared photothermal (MIP) approach reached 10 μM detection sensitivity and submicrometer lateral spatial resolution. This performance has exceeded the diffraction limit of infrared microscopy and allowed label-free three-dimensional chemical imaging of live cells and organisms. Distributions of endogenous lipid and exogenous drug inside single cells were visualized. We further demonstrated in vivo MIP imaging of lipids and proteins in
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.