ArticleBiomicrofluidics2016
Development of an advanced microfluidic micropipette aspiration device for single cell mechanics studies.
Article in Biomicrofluidics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- Viscoelastic recovery times of chondrocytes measured using a novel 3D-printed microfluidic device.Measurement science & technology · 2025Article
- Analytical methods in studying cell force sensing: principles, current technologies and perspectives.Regenerative biomaterials · 2025Review
- Mechanical deformation and death of circulating tumor cells in the bloodstream.Cancer metastasis reviews · 2024Review
- Cellular mechanotransduction of human osteoblasts in microgravity.NPJ microgravity · 2024Article
- Cellular mechanotransduction of human osteoblasts in microgravity.bioRxiv : the preprint server for biology · 2024Article
- Blood component separation in straight microfluidic channels.Biomicrofluidics · 2023Article
- A deformability-based biochip for precise label-free stratification of metastatic subtypes using deep learning.Microsystems & nanoengineering · 2023Article
- Microfluidic technologies for cell deformability cytometry.Smart medicine · 2022Review
- Biophysical Approaches for Applying and Measuring Biological Forces.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- The biophysics of cancer: emerging insights from micro- and nanoscale tools.Advanced nanobiomed research · 2022Article
- Simulating microgravity using a random positioning machine for inducing cellular responses to mechanotransduction in human osteoblasts.The Review of scientific instruments · 2021Article
- Non-invasive acquisition of mechanical properties of cells via passive microfluidic mechanisms: A review.Biomicrofluidics · 2021Review
- High-Throughput Cell Trapping in the Dentate Spiral Microfluidic Channel.Micromachines · 2021Article
- In-Situ Investigation on Nanoscopic Biomechanics ofInternational journal of molecular sciences · 2020Article
- Single-Cell Mechanophenotyping in Microfluidics to Evaluate Behavior of U87 Glioma Cells.Micromachines · 2020Article
- Mechanical characterization of vesicles and cells: A review.Electrophoresis · 2020Review
- On-chip surface acoustic wave and micropipette aspiration techniques to assess cell elastic properties.Biomicrofluidics · 2020Article
- Advances in Micropipette Aspiration: Applications in Cell Biomechanics, Models, and Extended Studies.Biophysical journal · 2019Review
- A Microfluidic Micropipette Aspiration Device to Study Single-Cell Mechanics Inspired by the Principle of Wheatstone Bridge.Micromachines · 2019Article
- Simultaneous monitoring of transcription and translation in mammalian cell-free expression in bulk and in cell-sized droplets.Synthetic biology (Oxford, England) · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Various micro-engineered tools or platforms have been developed recently for cell mechanics studies based on acoustic, magnetic, and optical actuations. Compared with other techniques for single cell manipulations, microfluidics has the advantages with simple working principles and device implementations. In this work, we develop a multi-layer microfluidic pipette aspiration device integrated with pneumatically actuated microfluidic control valves. This configuration enables decoupling of cell trapping and aspiration, and hence causes less mechanical perturbation on trapped single cells before aspiration. A high trapping efficiency is achieved by the microfluidic channel design based on fluid resistance model and deterministic microfluidics. Compared to conventional micropipette aspiration, the suction pressure applied on the aspirating cells is highly stable due to the viscous nature of low Reynolds number flow. As a proof-of-concept of this novel microfluidic technology, we built a microfluidic pipette aspiration device with 2 × 13 trapping arrays and used this device to measure the stiffness of a human breast cancer cell line, MDA-MB-231, through the observation of cell deformations during aspiration. As a comparison, we studied the effect of Taxol, a FDA-approved anticancer drug on single cancer cell stiffness. We found that cancer cells treated with Taxol were less deformable with a higher Young's modulus. The multi-layer microfluidic pipette aspiration device is a scalable technology for single cell mechanophenotyping studies and drug discovery applications.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.