ArticleScientific reports2016
Transcriptome and Metabolite analysis reveal candidate genes of the cardiac glycoside biosynthetic pathway from Calotropis procera.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 78 citations in OpenAlex.
- Chromosome scale assembly unveils genomic structure and gene families of Calotropis procera.Molecular genetics and genomics : MGG · 2025Article
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- Extreme temperatures elicit the accumulation of cardiac glycoside and their genin units in Calotropis procera by altering the expression of transcripts involved in its biosynthesis.Journal of plant research · 2025Article
- Genome and Tissue-Specific Transcriptome of the Tropical Milkweed (Plant direct · 2025Article
- Transcriptome analysis ofFrontiers in plant science · 2025Article
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- Four enzymes control natural variation in the steroid core ofbioRxiv : the preprint server for biology · 2024Article
- The Madagascar palm genome provides new insights on the evolution of Apocynaceae specialized metabolism.Heliyon · 2024Article
- Anti-Leishmania major activity of Calotropis procera extract by increasing ROS production and upregulating TNF-α, IFN-γ and iNOS mRNA expression under in vitro conditions.Tropical medicine and health · 2024Article
- Promiscuous CYP87A enzyme activity initiates cardenolide biosynthesis in plants.Nature plants · 2023Article
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- Bioactivities and in silico study of Pergularia tomentosa L. phytochemicals as potent antimicrobial agents targeting type IIA topoisomerase, TyrRS, and Sap1 virulence proteins.Environmental science and pollution research international · 2021Article
- 3D-surface MALDI mass spectrometry imaging for visualising plant defensive cardiac glycosides in Asclepias curassavica.Analytical and bioanalytical chemistry · 2021Article
- Systematic Phytochemical Screening of Different Organs ofMolecules (Basel, Switzerland) · 2021Article
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- Sequencing, de novo assembly and annotation of Digitalis ferruginea subsp. schischkinii transcriptome.Molecular biology reports · 2021Article
- Comparative transcriptome analysis of root, stem, and leaf tissues of Entada phaseoloides reveals potential genes involved in triterpenoid saponin biosynthesis.BMC genomics · 2020Article
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Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Calotropis procera is a medicinal plant of immense importance due to its pharmaceutical active components, especially cardiac glycosides (CG). As genomic resources for this plant are limited, the genes involved in CG biosynthetic pathway remain largely unknown till date. Our study on stage and tissue specific metabolite accumulation showed that CG's were maximally accumulated in stems of 3 month old seedlings. De novo transcriptome sequencing of same was done using high throughput Illumina HiSeq platform generating 44074 unigenes with average mean length of 1785 base pair. Around 66.6% of unigenes were annotated by using various public databases and 5324 unigenes showed significant match in the KEGG database involved in 133 different pathways of plant metabolism. Further KEGG analysis resulted in identification of 336 unigenes involved in cardenolide biosynthesis. Tissue specific expression analysis of 30 putative transcripts involved in terpenoid, steroid and cardenolide pathways showed a positive correlation between metabolite and transcript accumulation. Wound stress elevated CG levels as well the levels of the putative transcripts involved in its biosynthetic pathways. This result further validated the involvement of identified transcripts in CGs biosynthesis. The identified transcripts will lay a substantial foundation for further research on metabolic engineering and regulation of cardiac glycosides biosynthesis pathway genes.
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