ArticleTissue engineering. Part A2016
Calvarial Defect Healing Induced by Small Molecule Smoothened Agonist.
Article in Tissue engineering. Part A, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 25 citations in OpenAlex.
- Zein-Ceria Hybrid Microparticles Enable Long-Term ROS-Scavenging Oxygenation for Osteogenic Microtissues Engineering.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Effects of Smoothened Agonist Exposure on Murine Craniofacial Development.International dental journal · 2026Article
- Electrospun CHIR99021-loaded cellulose acetate scaffolds stimulate osteogenic differentiation and mineralization in vitro.Journal of materials science. Materials in medicine · 2026Article
- Hedgehog signaling pathway is an influential factor on vascular biology: a review.Journal of physiology and biochemistry · 2025Review
- Spatiotemporal application of small molecules in fracture healing.Biomaterials translational · 2025Review
- Bioactive Scaffolds as a Promising Alternative for Enhancing Critical-Size Bone Defect Regeneration in the Craniomaxillofacial Region.ACS applied bio materials · 2023Review
- Sequential application of small molecule therapy enhances chondrogenesis and angiogenesis in murine segmental defect bone repair.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2023Article
- Bone-Targeting Exosome Mimetics Engineered by Bioorthogonal Surface Functionalization for Bone Tissue Engineering.Nano letters · 2023Article
- BMP-6 promotes type 2 immune response during enhancement of rat mandibular bone defect healing.Frontiers in immunology · 2023Article
- From mesenchymal niches to engineeredMaterials today. Bio · 2022Article
- Hedgehog Signalling Contributes to Trauma-Induced Tendon Heterotopic Ossification and Regulates Osteogenesis through Antioxidant Pathway in Tendon-Derived Stem Cells.Antioxidants (Basel, Switzerland) · 2022Article
- Research progress on the hedgehog signalling pathway in regulating bone formation and homeostasis.Cell proliferation · 2022Review
- Divergent effects of distinct perivascular cell subsets for intra-articular cell therapy in posttraumatic osteoarthritis.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2021Article
- The Effects of Photobiomodulation on Bone Defect Repairing in a Diabetic Rat Model.International journal of molecular sciences · 2021Article
- Trb3 controls mesenchymal stem cell lineage fate and enhances bone regeneration by scaffold-mediated local gene delivery.Biomaterials · 2021Article
- Perivascular Fibro-Adipogenic Progenitor Tracing during Post-Traumatic Osteoarthritis.The American journal of pathology · 2020Article
- Smoothened agonist sterosome immobilized hybrid scaffold for bone regeneration.Science advances · 2020Article
- Article
- Skeletogenic Capacity of Human Perivascular Stem Cells Obtained Via Magnetic-Activated Cell Sorting.Tissue engineering. Part A · 2019Article
- Assessment of Hedgehog Signaling Pathway Activation for Craniofacial Bone Regeneration in a Critical-Sized Rat Mandibular Defect.JAMA facial plastic surgery · 2019Article
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
Abstract
Hedgehog (Hh) signaling positively regulates both endochondral and intramembranous ossification. Use of small molecules for tissue engineering applications poses several advantages. In this study, we examined whether use of an acellular scaffold treated with the small molecule Smoothened agonist (SAG) could aid in critical-size mouse calvarial defect repair. First, we verified the pro-osteogenic effect of SAG in vitro, using primary neonatal mouse calvarial cells (NMCCs). Next, a 4 mm nonhealing defect was created in the mid-parietal bone of 10-week-old CD-1 mice. The scaffold consisted of a custom-fabricated poly(lactic-co-glycolic acid) disc with hydroxyapatite coating (measuring 4 mm diameter × 0.5 mm thickness). Treatment groups included dimethylsulfoxide control (n = 6), 0.5 mM SAG (n = 7) or 1.0 mM SAG (n = 7). Evaluation was performed at 4 and 8 weeks postoperative, by a combination of high-resolution microcomputed tomography, histology (H & E, Masson's Trichrome), histomorphometry, and immunohistochemistry (BSP, OCN, VEGF). In vivo results showed that SAG treatment induced a significant and dose-dependent increase in calvarial bone healing by all radiographic parameters. Histomorphometric analysis showed an increase in all parameters of bone formation with SAG treatment, but also an increase in blood vessel number and density. In summary, SAG is a pro-osteogenic, provasculogenic stimulus when applied locally in a bone defect environment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.