ArticlePloS one2016
Protocadherin-1 Localization and Cell-Adhesion Function in Airway Epithelial Cells in Asthma.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 29 citations in OpenAlex.
- The involvement of Th17 inflammation and miR-363-3p in airway epithelial barrier dysfunction.Respiratory research · 2026Article
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- Optimization of Methods for the Quantitative Analysis of Global Cell Surface Proteome and Cell Surface Polarization.International journal of molecular sciences · 2025Article
- Knockdown of AMIGO2 suppresses proliferation and migration through regulating PPAR-γ in bladder cancer.Hereditas · 2024Article
- Insight into the Interplay of Gd-IgA1, HMGB1, RAGE and PCDH1 in IgA Vasculitis (IgAV).International journal of molecular sciences · 2024Article
- Mapping combinatorial expression of non-clustered protocadherins in the developing brain identifies novel PCDH19-mediated cell adhesion properties.Open biology · 2024Article
- Barrier Impairment and Type 2 Inflammation in Allergic Diseases: The Pediatric Perspective.Children (Basel, Switzerland) · 2021Review
- Article
- Modulation of the EMT/MET Process by E-Cadherin in Airway Epithelia Stress Injury.Biomolecules · 2021Article
- Review
- Epithelial barriers in allergy and asthma.The Journal of allergy and clinical immunology · 2020Review
- Modifying and IntegratingFrontiers in bioengineering and biotechnology · 2020Review
- Protocadherins at the Crossroad of Signaling Pathways.Frontiers in molecular neuroscience · 2020Review
- Pathway analysis of a genome-wide gene by air pollution interaction study in asthmatic children.Journal of exposure science & environmental epidemiology · 2019Article
- Pathway centrality in protein interaction networks identifies putative functional mediating pathways in pulmonary disease.Scientific reports · 2019Article
- Identification of an adhesive interface for the non-clustered δ1 protocadherin-1 involved in respiratory diseases.Communications biology · 2019Article
- Hantavirus entry: Perspectives and recent advances.Advances in virus research · 2019Article
- Role of PCDH 1 Gene in the Development of Childhood Asthma and Other Related Phenotypes: A Literature Review.Cureus · 2018Review
- Hypoxia-derived exosomes induce putative altered pathways in biosynthesis and ion regulatory channels in glioblastoma cells.Biochemistry and biophysics reports · 2018Article
Corrections and comments
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Authors and funding
17 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe asthma gene PCDH1 encodes Protocadherin-1, a putative adhesion molecule of unknown function expressed in the airway epithelium. Here, we characterize the localization, differential expression, homotypic adhesion specificity and function of PCDH1 in airway epithelial cells in asthma.
methodsWe performed confocal fluorescence microscopy to determine subcellular localization of PCDH1 in 16HBE cells and primary bronchial epithelial cells (PBECs) grown at air-liquid interface. Next, to compare PCDH1 expression and localization in asthma and controls we performed qRT-PCR and fluorescence microscopy in PBECs and immunohistochemistry on airway wall biopsies. We examined homotypic adhesion specificity of HEK293T clones overexpressing fluorescently tagged-PCDH1 isoforms. Finally, to evaluate the role for PCDH1 in epithelial barrier formation and repair, we performed siRNA knockdown-studies and measured epithelial resistance.
resultsPCDH1 localized to the cell membrane at cell-cell contact sites, baso-lateral to adherens junctions, with increasing expression during epithelial differentiation. No differences in gene expression or localization of PCDH1 isoforms expressing the extracellular domain were observed in either PBECs or airway wall biopsies between asthma patients and controls. Overexpression of PCDH1 mediated homotypic interaction, whereas downregulation of PCDH1 reduced epithelial barrier formation, and impaired repair after wounding.
conclusionsIn conclusion, PCDH1 is localized to the cell membrane of bronchial epithelial cells baso-lateral to the adherens junction. Expression of PCDH1 is not reduced nor delocalized in asthma even though PCDH1 contributes to homotypic adhesion, epithelial barrier formation and repair.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.