ArticleBiomedical optics express2016
3D imaging in CUBIC-cleared mouse heart tissue: going deeper.
Article in Biomedical optics express, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Advanced imaging strategies in cardiac organoids: bridging the gap between structural complexity and functional analysis.Cardiovascular diabetology · 2026Review
- Immersion-Based Clearing and Autofluorescence Quenching in Myocardial Tissue.Microcirculation (New York, N.Y. : 1994) · 2025Article
- Evaluation of the effects of clearing agents, fixation, and process durations on cardiovascular tissue imaging with second harmonic generation and multi-photon modalities.Frontiers in bioengineering and biotechnology · 2025Article
- Quantifying Microvascular Structure in Healthy and Infarcted Rat Hearts Using Optical Coherence Tomography Angiography.IEEE transactions on medical imaging · 2024Article
- Successful 3D imaging of cleared biological samples with light sheet fluorescence microscopy.The Journal of cell biology · 2023Review
- Vacuum-assisted tissue embedding for whole-heart imaging.Biomedical optics express · 2023Article
- Visualization of Organ-Specific Lymphatic Growth: An Efficient Approach to Labeling Molecular Markers in Cleared Tissues.International journal of molecular sciences · 2023Article
- A 3D Analysis of Cleared Human Melanoma.Biomedicines · 2022Article
- Tissue Optical Clearing: State of the Art and Prospects.Diagnostics (Basel, Switzerland) · 2022Article
- Optical tissue clearing associated with 3D imaging: application in preclinical and clinical studies.Histochemistry and cell biology · 2022Review
- It's clearly the heart! Optical transparency, cardiac tissue imaging, and computer modelling.Progress in biophysics and molecular biology · 2022Article
- Refined CLARITY-Based Tissue Clearing for Three-Dimensional Fibroblast Organization in Healthy and Injured Mouse Hearts.Journal of visualized experiments : JoVE · 2021Article
- Review
- Spatial topological analysis of sympathetic neurovascular characteristic of acupoints in Ren meridian using advanced tissue-clearing and near infrared II imaging.Computational and structural biotechnology journal · 2021Article
- Biomedical Applications of Tissue Clearing and Three-Dimensional Imaging in Health and Disease.iScience · 2020Review
- Impact of optical tissue clearing on the Brillouin signal from biological tissue samples.Biomedical optics express · 2019Article
- Identification of peripheral neural circuits that regulate heart rate using optogenetic and viral vector strategies.Nature communications · 2019Article
- Imaging the brain in 3D using a combination of CUBIC and immunofluorescence staining.Biomedical optics express · 2019Article
- A Novel Optical Tissue Clearing Protocol for Mouse Skeletal Muscle to Visualize Endplates in Their Tissue Context.Frontiers in cellular neuroscience · 2019Article
- Applications of Light-Sheet Microscopy in Microdevices.Frontiers in neuroanatomy · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to acquire high resolution 3D images of the heart enables to study heart diseases more in detail. In this work, the CUBIC (clear, unobstructed brain imaging cocktails and computational analysis) clearing protocol was optimized for thick mouse heart sections to enhance the penetration depth of the confocal microscope lasers into the tissue. In addition, the optimized CUBIC clearing of the heart enhances antibody penetration into the tissue by a factor of five. The present protocol enables deep 3D high-quality image acquisition in the heart allowing a much more accurate assessment of the cellular and structural changes that underlie heart diseases.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.