Evidence map›Paper›PMID 27699129›Full record

ArticleBiomedical optics express2016

Motion quantification during multi-photon functional imaging in behaving animals.

Lingjie Kong, Justin P Little, Meng Cui

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Article
  3. Fast wavefront shaping for two-photon brain imaging with multipatch correction.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  4. Article
  5. Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Lingjie KongSchool of Electrical and Computer Engineering, Purdue University, West Lafayette, IN 47907, USA; kong52@purdue.edu.
Justin P LittleJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 19700, USA.
Meng CuiSchool of Electrical and Computer Engineering, Purdue University, West Lafayette, IN 47907, USA; Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA; Integrated Imaging Cluster, Purdue University, West Lafayette, IN 47907, USA; Bindley Bioscience Center, Purdue University, West Lafayette, IN 47907, USA; mengcui@purdue.edu.

Funding

Super-resolution deep tissue imaging of dendritic spinesU01NS094341 · NINDS · PURDUE UNIVERSITY · PI CUI, MENG, GAN, WENBIAO · 2015 to 2017
$1.5M
NINDS NIH HHS U01 NS094341
6 · The paper itself

Abstract

Functional imaging in behaving animals is essential to understanding brain function. However, artifacts resulting from animal motion, including locomotion, can severely corrupt functional measurements. To dampen tissue motion, we designed a new optical window with minimal optical aberrations. Using the newly developed high-speed continuous volumetric imaging system based on an optical phase-locked ultrasound lens, we quantified motion of the cerebral cortex and hippocampal surface during two-photon functional imaging in behaving mice. We find that the out-of-plane motion is generally greater than the axial dimension of the point-spread-function during mouse locomotion, which indicates that high-speed continuous volumetric imaging is necessary to minimize motion artifacts.

Indexed as

(170.0170) Medical optics and biotechnology(170.3880) Medical and biological imaging(180.2520) Fluorescence microscopy(180.4315) Nonlinear microscopy

Identifiers

PMID27699129
PMCPMC5030041

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.